FA/VB9 (Folic Acid/Vitamin B9) BioAssay™ ELISA Kit (Universal)

The FA/VB9 (Folic Acid/Vitamin B9) BioAssay™ ELISA Kit is a quantitative competitive assay for the detection of Vitamin B12 in serum, plasma, tissue homogenates and other biological fluids.

12.5-800ng/ml

<7.5ng/ml

Intra-assay CV: <8%
Inter-assay CV: <10%

This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to FA/VB9. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to FA/VB9. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FA/VB9, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FA/VB9 in the sample is then determined by an inverse correlation between FA/VB9 in the samples and the assay signal intensity.

*355547A: Microtiter Strips, 1x96 wells (8x12 wells).
*355547B: Standard, 2x1 vials
355547C: Sample/Standard Dilution Buffer, 1x20ml
355547D: Antibody (Biotin) (Concentrated), 1x60ul
355547E: Antibody Dilution Buffer, 1x10ml
355547F: Streptavidin (HRP) (SABC), 1x120ul
355547G: SABC Dilution Buffer, 1x10ml
355547H: TMB Substrate, 1x10ml
355547J: Stop Solution, 1x10ml
355547K: Wash Buffer, 25X, 1x30ml

Store unopened *355547A at 4ºC; store at -20ºC once opened. Store unopened *355547B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

1. Wash plate 2 times before adding standards, samples and control (zero) to wells!
2. Add 50ul standard or sample to each well. Immediately add 50ul (Biotin) working solution to each well.
3. Incubate for 45 minutes at 37°C
4. Aspirate and wash 3 times.
5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C
6. Aspirate and wash 5 times.
7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
9. Calculate results.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

 All products should be handled by qualified personnel only, trained in laboratory procedures.