Epinephrine (EPI) BioAssay™ ELISA Kit (All Species)
Cat# 024859-96T
Size : 96Tests
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
Shipping Temp
Blue IceStorage Temp
4°C/-20°CThe Epinephrine (EPI) BioAssay™ ELISA Kit (All Species) is a competitive inhibition immunoassay for the in vitro quantitative measurement of EPI in plasma, serum, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Detection Range:
24.69-2,000pg/ml
Sensitivity:
<9.74pg/ml
Precision:
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Inter-Assay CV: <12%
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for EPI has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled EPI and unlabeled EPI (standards or samples) for limited binding sites on the pre-coated antibody. After incubation, unbound materials are washed off. Next, avidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of EPI in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of EPI in the sample.
Kit Components:
*024859A: Microtiter Plate, 96 wells, Pre-coated, ready to use.
*024859B: Standard, 2x1vial
024859C: Standard Diluent, 1x20ml
*024859D: Detection Reagent A, 1x120ul
*024859E: Detection Reagent B, 1x120ul
024859F: Assay Diluent A, 1x12ml
024859G: Assay Diluent B, 1x12ml
024859H: TMB Substrate, 1x9ml
024859K: Stop Solution, 1x6ml
024859L: Wash Buffer, 30X, 1x20ml
*024859B: Standard, 2x1vial
024859C: Standard Diluent, 1x20ml
*024859D: Detection Reagent A, 1x120ul
*024859E: Detection Reagent B, 1x120ul
024859F: Assay Diluent A, 1x12ml
024859G: Assay Diluent B, 1x12ml
024859H: TMB Substrate, 1x9ml
024859K: Stop Solution, 1x6ml
024859L: Wash Buffer, 30X, 1x20ml
Storage and Stability:
Store *024859A, *024859B, *024859D and *024859E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Materials Required But Not Supplied:
1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution
Assay Summary:
1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
2. Add 50ul standard or sample to each well, then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.