EnzyChrom™ Phospholipid Assay Kit
Application
- For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.
Key Features
- Sensitive. Use 20 µL samples. Linear detection range: colorimetric assay 3 – 200 µM, fluorimetric assay 0.6 – 20 µM phospholipid.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD570nm, or FL530/585nm
Samples
- Biological
Species
- All
Procedure
- 20 min
Size
- 100 tests
Detection Limit
- OD, FL: 3, 0.6 µM
Shelf Life
- 6 months
More Details
Phospholipids are a class of lipids that constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. BioAssay Systems method provides a simple, direct, and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink-colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.I am looking for assay to measure phospholipids in cell culture media. I think the concentration in media maybe much lower than the serum. I am not sure whether if this kit is suitable for me.
BioAssay Systems’ phospholipid assay kit has a linear detection range from 3 – 200 μM (colorimetric assay), and 0.6 – 20 μM (fluorimetric assay). We have not tested cell culture media, but believe it should work in most cases. Common media formulations will contain about 5-10% FCS and the phospholipid content therein should be in the linear detection range of the assay. Other media formulations may have much lower serum concentrations or are serum-free. In those cases you should contact the manufacturer to get an estimate of how much phospholipid is in your medium.
Robuck, AR et al. (2020). Legacy and novel per- and polyfluoroalkyl substances in juvenile seabirds from the u. S. Atlantic coast. Environmental Science & Technology, 54(20): 12938-12948. Assay: Phospholipid in avian liver.
Kim, SH et al. (2019). The early onset and persistent worsening pulmonary alveolar proteinosis in rats by indium oxide nanoparticles. Nanotoxicology: 1-11. Assay: Phospholipid in rat bronchoalveolar lavage fluid.
Lee, DK et al. (2020). Aggravation of atherosclerosis by pulmonary exposure to indium oxide nanoparticles. Nanotoxicology: 1-17. Assay: Phospholipid in rat bronchoalveolar lavage fluid.
Chi, Y et al. (2020). PHTF2 regulates lipids metabolism in gastric cancer. Aging (Albany NY), 12(8): 6600-6610. Assay: Phospholipid in human cells.
Dai, W et al. (2020). OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. Journal of Hepatology, 72(5): 909-923. Assay: Phospholipid in human liver cells.
Dassuncao, C., Pickard, H., Pfohl, M., Tokranov, A. K., Li, M., Mikkelsen, B., & Sunderland, E. M. (2019). Phospholipid Levels Predict the Tissue Distribution of Poly-and Perfluoroalkyl Substances in a Marine Mammal. Environ Sci Tech Let, 6(3), 119-125. Assay: Phospholipid in human tissues.
Siew, S. M., Cunningham, S. C., Zhu, E., Tay, S. S., Venuti, E., Bolitho, C., & Alexander, I. E. (2019). Prevention of cholestatic liver disease and reduced tumorigenicity in a murine model of PFIC type 3 using hybrid AAV-piggyBac gene therapy. Hepatology, doi:10.1002/hep.30773 Assay: Phospholipid in rat Liver tissue.
Badylak, S. F., & Keane Jr, T. J. (2018). Methods of preparing ecm scaffolds and hydrogels from colon. U.S. Patent Application No. 15/742,676. Assay: Phospholipid in human intestinal epithelial cells.
Bhattacharjee, P., & Keyel, P. A. (2018). Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Scientific reports, 8(1), 6458. Assay: Phospholipid in murine bone marrow derived macrophages.
Alexander, I., Cunningham, S., & Nagy, A. (2017). Stable Gene Transfer to Proliferating Cells. U.S. Patent Application No. 15/127,743. Assay: Phospholipid in monkey bile.
Keane, T. J., Dziki, J., Castelton, A., Faulk, D. M., Messerschmidt, V., Londono, R., & Badylak, S. F. (2017). Preparation and characterization of a biologic scaffold and hydrogel derived from colonic mucosa. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 105(2), 291-306. Assay: Phospholipid in pig colon.
Yang, W., Huang, L., Gao, J., Wen, S., Tai, Y., Chen, M., & Li, J. (2017). Betaine attenuates chronic alcohol-induced fatty liver by broadly regulating hepatic lipid metabolism. Molecular medicine reports, 16(4), 5225-5234. Assay: Phospholipid in rat hepatocytes, serum.
Jeong, J., Kim, J., Seok, S. H., & Cho, W. S. (2016). Indium oxide (In 2 O 3) nanoparticles induce progressive lung injury distinct from lung injuries by copper oxide (CuO) and nickel oxide (NiO) nanoparticles. Archives of toxicology, 90(4), 817-828. Assay: Phospholipid in mouse.
Dhamdhere, GR et al (2014). Drugging a stem cell compartment using Wnt3a protein as a therapeutic. PloS one 9.1: e83650. Assay: Phospholipid in synthetic liposome.
Goktas, S et al (2014). Decellularization method influences early remodeling of an allogenic tissue scaffold. Journal of Biomedical Materials Research Part A102.1: 8-16. Assay: Phospholipid in human umbilical cord tissue.
Tan, LN et al (2014). Liquid crystal droplet-based amplification of microvesicles that are shed by mammalian cells. Analyst. Assay: Phospholipid in human epidermoid cancer cell line.
Gong, J et al (2013). Microparticle drug sequestration provides a parallel pathway in the acquisition of cancer drug resistance. European journal of pharmacology 721.1: 116-125. Assay: Phospholipid in synthetic microparticles.
Kaszas, N et al (2013). Ciprofloxacin encapsulation into giant unilamellar vesicles: Membrane binding and release. Journal of pharmaceutical sciences 102.2: 694-705. Assay: Phospholipid in synthetic vesicles.
Haynes TA et al (2012). DNA damage induces down-regulation of UDP-glucose ceramide glucosyltransferase, increases ceramide levels and triggers apoptosis in p53-deficient cancer cells. Biochim Biophys Acta 1821(7):943-53. Assay: Phospholipid in human Ovarian cancer cells.
Kim S et al (2012) Aberrant upregulation of astroglial ceramide potentiates oligodendrocyte injury. Brain Pathol 22(1):41-47. Assay: Phospholipid in lipid.
Mukherjee, A et al (2012). Lysophosphatidic acid activates lipogenic pathways and de novo lipid synthesis in ovarian cancer cells. Journal of Biological Chemistry 287.30: 24990-25000. Assay: Phospholipid in human ovarian cancer cell line.
Soga N et al (2012) Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis. J Biol Chem 287(12):9633-9. Assay: Phospholipid in human cancer cell.
Stoll C et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-481. Assay: Phospholipid in mouse brain.
Stoll C, et al (2011). Liposomes alter thermal phase behavior and composition of red blood cell membranes. Biochim Biophys Acta 1808(1):474-81. Assay: Phospholipid in human red blood cell.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost