EnzyChrom™ NADP/NADPH Assay Kit

Cat# E2NP-100

Size : 100tests

Brand : BioAssay Systems

Contact local distributor :

Phone : +1 850 650 7790

EnzyChrom™ NADP+/NADPH Assay Kit


  • For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts.

Key Features

  • Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.


  • Colorimetric Determination at 565 nm


  • Cells, Tissue


  • human, mouse, rat, and more


  • 30 min


  • 100 tests

Detection Limit

  • 0.1 µM

Shelf Life

  • 6 months

More Details

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue. Simple, direct, and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems’ EnzyChromTM NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered with by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH, and their ratio.

When tissue is used, should it be freshly obtained? Or is -80°C storage ok?

If direct sample processing is not possible, we recommend snap freezing tissue samples in liquid nitrogen and keeping them either in liquid nitrogen or at -80°C until further processing.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Catalog No: E2NP-100 Categories Blood & Urine Chemistry, Metabolism, Oxidative Stress Tags Blood & Urine Chemistry, E2NP-100, EnzyChrom NADP+/NADPH Assay Kits, EnzyChromTM NADP+/NADPH Assay Kit, metabolism, NADP+/NADPH assay kits, NADP+/NADPH determination kits, NADP+/NADPH measurement kits, NADP+/NADPH test kits, Oxidative Stress

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

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