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Reagents and instruments for immunology, cell biology and molecular biology.

Docetaxel (Trihydrate) [148408-66-6]

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Cat# HY-B0011A-100mg

Size : 100mg

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Description

Docetaxel Trihydrate (RP-56976 Trihydrate) is an antineoplastic agent and inhibits microtubule depolymerization with an IC₅₀ value of 0.2 μM^[1]. Docetaxel Trihydrate is a semisynthetic analog of taxol and attenuates the effects of bcl-2 and bcl-xL gene expression. Docetaxel Trihydrate arrests the cell cycle at G2/M and leads to cell apoptosis^[1]^[3].

IC₅₀ & Target

Microtubule^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A121	IC₅₀	1.2 nM Compound: Docetaxel	Inhibition of the growth against human Ovarian Carcinoma (A121) cell line after 72 hr exposure Inhibition of the growth against human Ovarian Carcinoma (A121) cell line after 72 hr exposure	[PMID: 9022794]
A2780	IC₅₀	0.6 nM Compound: 1b	Cytotoxicity against human A2780 cells after 48 hrs by MTT assay Cytotoxicity against human A2780 cells after 48 hrs by MTT assay	[PMID: 19128972]
A2780	IC₅₀	0.6 nM Compound: Docetaxel	Cytotoxicity against human A2780 cells by MTT assay Cytotoxicity against human A2780 cells by MTT assay	[PMID: 25047938]
A2780	IC₅₀	126 nM Compound: Docetaxel	Antiproliferative against multidrug-resistant human A2780 cells after 72 hrs by MTT assay Antiproliferative against multidrug-resistant human A2780 cells after 72 hrs by MTT assay	[PMID: 24835987]
A2780	IC₅₀	290 nM Compound: 1b	Cytotoxicity against p-glycoprotein expressing drug-resistant human A2780 cells after 48 hrs by MTT assay Cytotoxicity against p-glycoprotein expressing drug-resistant human A2780 cells after 48 hrs by MTT assay	[PMID: 19128972]
A2780	IC₅₀	33 nM Compound: Docetaxel	Antiproliferative against human A2780 cells after 72 hrs by MTT assay Antiproliferative against human A2780 cells after 72 hrs by MTT assay	[PMID: 24835987]
A2780 ADR	IC₅₀	290 nM Compound: Docetaxel	Cytotoxicity against human A2780AD cells by MTT assay Cytotoxicity against human A2780AD cells by MTT assay	[PMID: 25047938]
A549	IC₅₀	> 1.4 μg/mL Compound: docetaxel	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 17624790]
A549	IC₅₀	0.37 μM Compound: Taxotere	Cytotoxicity against human A549 cells after 72 hrs Cytotoxicity against human A549 cells after 72 hrs	[PMID: 20849074]
A549	IC₅₀	1 nM Compound: 1b Docetaxel	Compound was tested for its cytotoxicity by inhibiting the growth of A-549 cell line after 72 h Compound was tested for its cytotoxicity by inhibiting the growth of A-549 cell line after 72 h	[PMID: 15225730]
A549	IC₅₀	1 nM Compound: Docetaxel	Inhibition of the growth against human non-small-lung carcinoma (A549) cell line after 72 hr exposure Inhibition of the growth against human non-small-lung carcinoma (A549) cell line after 72 hr exposure	[PMID: 9022794]
A549	IC₅₀	1 nM Compound: Docetaxel	In vitro cytotoxicity of compound against human A549 (non small cell lung) cancer cell line after 72 hr of drug exposure In vitro cytotoxicity of compound against human A549 (non small cell lung) cancer cell line after 72 hr of drug exposure	[PMID: 12477344]
A549	IC₅₀	1 μM Compound: 2	inhibitory concentration against the growth of Non-small-cell lung carcinoma A549 cell line after 72 hr of exposure inhibitory concentration against the growth of Non-small-cell lung carcinoma A549 cell line after 72 hr of exposure	[PMID: 15501062]
A549	IC₅₀	1.7 μM Compound: docetaxel	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 18226907]
A549	IC₅₀	27.256 μg/mL Compound: Docetaxel	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
A549	IC₅₀	35.2 nM Compound: Docetaxel	Antiproliferative against human A549 cells after 72 hrs by MTT assay Antiproliferative against human A549 cells after 72 hrs by MTT assay	[PMID: 24835987]
A549	IC₅₀	4.95 x 10^-4 μM Compound: docetaxel	Cytotoxicity against human A549 cells after 96 hrs by microplate reader assay Cytotoxicity against human A549 cells after 96 hrs by microplate reader assay	[PMID: 19133778]
A549	IC₅₀	850 nM Compound: 2, DTX, Taxotere	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 24269481]
Bel-7402	IC₅₀	1.9 nM Compound: Docetaxel	Antiproliferative activity against human Bel7402 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human Bel7402 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
C6	IC₅₀	1 nM Compound: docetaxel	Cytotoxicity against Pgp deficient human LCC6 cells after 72 hrs by sulforhodamine B test Cytotoxicity against Pgp deficient human LCC6 cells after 72 hrs by sulforhodamine B test	[PMID: 18465846]
Calu-3	IC₅₀	0.1 μM Compound: docetaxel	Cytotoxicity against human Calu3 cells after 72 hrs by SRB assay Cytotoxicity against human Calu3 cells after 72 hrs by SRB assay	[PMID: 19028425]
COLO 205	IC₅₀	30.706 μg/mL Compound: Docetaxel	Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
HCC1806	IC₅₀	8.4 μM Compound: docetaxel	Cytotoxicity against human HCC1806 cells after 48 hrs by MTT assay Cytotoxicity against human HCC1806 cells after 48 hrs by MTT assay	[PMID: 22560315]
HCT-116	IC₅₀	0.0022 μM Compound: 1b	Concentration required for inhibition of 50% human colon HCT 116 cell line proliferation after 72 hr of incubation Concentration required for inhibition of 50% human colon HCT 116 cell line proliferation after 72 hr of incubation	[PMID: 15537348]
HCT-116	IC₅₀	0.18 nM Compound: 7	Antiproliferative activity against IC95 biaryl selected drug-resistant against human HCT116 cells after 72 hrs by Cell titer Glo assay Antiproliferative activity against IC95 biaryl selected drug-resistant against human HCT116 cells after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-116	IC₅₀	0.2 nM Compound: 7	Antiproliferative activity against IC50 biaryl selected human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay Antiproliferative activity against IC50 biaryl selected human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-116	IC₅₀	0.23 nM Compound: 7	Antiproliferative activity against human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay Antiproliferative activity against human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-116	IC₅₀	0.3 nM Compound: 7	Antiproliferative activity against human HCT116 cells after 72 hrs by Cell titer Glo assay Antiproliferative activity against human HCT116 cells after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-116	IC₅₀	0.31 nM Compound: 7	Antiproliferative activity against IC50 biaryl selected drug-resistant against human HCT116 cells after 72 hrs by Cell titer Glo assay Antiproliferative activity against IC50 biaryl selected drug-resistant against human HCT116 cells after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-116	IC₅₀	0.35 nM Compound: 7	Antiproliferative activity against 3x IC95 biaryl selected human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay Antiproliferative activity against 3x IC95 biaryl selected human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-116	IC₅₀	0.5 nM Compound: docetaxel	Cytotoxicity against human HCT116 cells after 72 hrs by MTS assay Cytotoxicity against human HCT116 cells after 72 hrs by MTS assay	[PMID: 19655762]
HCT-116	IC₅₀	0.52 nM Compound: taxotere	Cytotoxicity against human HCT116 cells incubated for 72 hrs by MTS assay Cytotoxicity against human HCT116 cells incubated for 72 hrs by MTS assay	[PMID: 23072299]
HCT-116	IC₅₀	0.52 nM Compound: Taxotere	Cytotoxicity against human HCT116 cells after 72 hrs by MTS assay Cytotoxicity against human HCT116 cells after 72 hrs by MTS assay	[PMID: 21142180]
HCT-116	IC₅₀	0.59 nM Compound: 7	Antiproliferative activity against IC95 biaryl selected human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay Antiproliferative activity against IC95 biaryl selected human HCT116 cells expressing Kinesin-like protein KIF11 D130V mutant after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-116	IC₅₀	3 nM Compound: Docetaxel	In vitro inhibition of proliferation of human colon cancer line 116 after 72 hrs incubation. In vitro inhibition of proliferation of human colon cancer line 116 after 72 hrs incubation.	10.1016/S0960-894X(00)80075-X
HCT-116	IC₅₀	5 x 10^-2 μM Compound: Taxotere	Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay	[PMID: 23387796]
HCT-116	IC₅₀	8.7 nM Compound: 7	Antiproliferative activity against 3x IC95 biaryl selected drug-resistant against human HCT116 cells after 72 hrs by Cell titer Glo assay Antiproliferative activity against 3x IC95 biaryl selected drug-resistant against human HCT116 cells after 72 hrs by Cell titer Glo assay	[PMID: 17922005]
HCT-15	IC₅₀	13 nM Compound: docetaxel	Cytotoxicity against human HCT15 cells after 72 hrs by MTS assay Cytotoxicity against human HCT15 cells after 72 hrs by MTS assay	[PMID: 19655762]
HeLa	IC₅₀	50.1 nM Compound: Docetaxel	Antiproliferative against human HeLa cells after 72 hrs by MTT assay Antiproliferative against human HeLa cells after 72 hrs by MTT assay	[PMID: 24835987]
Hep 3B2	IC₅₀	38.2 nM Compound: Docetaxel	Antiproliferative activity against human Hep3B after 72 hrs by CellTiter Glo assay Antiproliferative activity against human Hep3B after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
HepG2	IC₅₀	> 100 nM Compound: Docetaxel	Antiproliferative activity against human HepG2 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human HepG2 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
HepG2	IC₅₀	> 400 μM Compound: Taxotere	Cytotoxicity against human HepG2 cells by SRB method Cytotoxicity against human HepG2 cells by SRB method	[PMID: 18181575]
HL-60	IC₅₀	0.5 nM Compound: taxotere	Cytotoxicity against human HL60 cells incubated for 72 hrs by MTS assay Cytotoxicity against human HL60 cells incubated for 72 hrs by MTS assay	[PMID: 23072299]
HL-60	IC₅₀	0.5 nM Compound: Taxotere	Cytotoxicity against human HL60 cells after 72 hrs by MTS assay Cytotoxicity against human HL60 cells after 72 hrs by MTS assay	[PMID: 21142180]
HL-60	IC₅₀	0.5 nM Compound: docetaxel	Cytotoxicity against human HL60 cells after 72 hrs by MTS assay Cytotoxicity against human HL60 cells after 72 hrs by MTS assay	[PMID: 19655762]
HL-60	IC₅₀	0.52 μM Compound: Taxotere	Cytotoxicity against human HL60 cells after 72 hrs Cytotoxicity against human HL60 cells after 72 hrs	[PMID: 20849074]
HL-60	IC₅₀	531 nM Compound: Taxotere	Cytotoxicity against human HL60R cells after 72 hrs by MTS assay Cytotoxicity against human HL60R cells after 72 hrs by MTS assay	[PMID: 21142180]
HT-29	IC₅₀	0.92 μM Compound: Taxotere	Cytotoxicity against human HT-29 cells after 72 hrs Cytotoxicity against human HT-29 cells after 72 hrs	[PMID: 20849074]
HT-29	IC₅₀	1 nM Compound: docetaxel	Cytotoxicity against human HT-29 cells after 72 hrs Cytotoxicity against human HT-29 cells after 72 hrs	[PMID: 19239240]
HT-29	IC₅₀	1.2 nM Compound: Docetaxel	Inhibition of the growth against human Colon Carcinoma (HT-29) cell line after 72 hr exposure Inhibition of the growth against human Colon Carcinoma (HT-29) cell line after 72 hr exposure	[PMID: 9022794]
HT-29	IC₅₀	3.34 x 10^-4 μM Compound: docetaxel	Cytotoxicity against human HT-29 cells after 96 hrs by microplate reader assay Cytotoxicity against human HT-29 cells after 96 hrs by microplate reader assay	[PMID: 19133778]
Huh-7	IC₅₀	12.1 nM Compound: Docetaxel	Antiproliferative activity against human HuH7 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human HuH7 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
IMR-32	IC₅₀	23.71 μg/mL Compound: Docetaxel	Cytotoxicity against human IMR32 cells after 72 hrs by MTT assay Cytotoxicity against human IMR32 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
J774	ED₅₀	0.05 μM Compound: 3a (Taxotere 10-OH)	In vitro cytotoxicity against J774.2 cells after 72 hr incubation. In vitro cytotoxicity against J774.2 cells after 72 hr incubation.	10.1016/S0960-894X(01)80527-8
K562	IC₅₀	> 400 μM Compound: Taxotere	Cytotoxicity against human K562 cells by SRB method Cytotoxicity against human K562 cells by SRB method	[PMID: 18181575]
K562	IC₅₀	6.9 μg/mL Compound: docetaxel	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 17624790]
K562	IC₅₀	8.5 μM Compound: docetaxel	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 18226907]
KB	IC₅₀	0.0001 μM Compound: docetaxel, 1b	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 17064914]
KB	IC₅₀	0.0002 μM Compound: taxotere	Cytotoxicity against human KB cells after 72 hrs by neutral red staining Cytotoxicity against human KB cells after 72 hrs by neutral red staining	[PMID: 19053513]
KB	IC₅₀	0.00025 μM Compound: Taxotere	Cytotoxicity against human KB cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay Cytotoxicity against human KB cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay	[PMID: 23387796]
KB	IC₅₀	0.0003 μM Compound: 1b	Concentration required for inhibition of 50% human fibroblast KB cell line proliferation after 72 hr of incubation Concentration required for inhibition of 50% human fibroblast KB cell line proliferation after 72 hr of incubation	[PMID: 15537348]
KB	IC₅₀	0.15 nM Compound: Taxotere	Cytotoxicity against human KB cells after 72 hrs by neutral red based colorimetry assay Cytotoxicity against human KB cells after 72 hrs by neutral red based colorimetry assay	[PMID: 21443172]
KB	IC₅₀	0.17 nM Compound: taxotere	Cytotoxicity against human KB cells incubated for 72 hrs by MTS assay Cytotoxicity against human KB cells incubated for 72 hrs by MTS assay	[PMID: 23072299]
KB	IC₅₀	0.17 nM Compound: Taxotere	Cytotoxicity against human KB cells after 72 hrs by MTS assay Cytotoxicity against human KB cells after 72 hrs by MTS assay	[PMID: 21142180]
KB	IC₅₀	0.2 nM Compound: docetaxel	Cytotoxicity against human KB cells after 72 hrs by MTS assay Cytotoxicity against human KB cells after 72 hrs by MTS assay	[PMID: 19655762]
KB	IC₅₀	0.29 μM Compound: Taxotere	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 20849074]
KB	IC₅₀	0.4 nM Compound: Taxotere	Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by MTS assay Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by MTS assay	[PMID: 20053482]
KB	IC₅₀	1.14 nM Compound: Taxotere	Cytotoxicity against human KB cells by SRB method Cytotoxicity against human KB cells by SRB method	[PMID: 18181575]
KB	IC₅₀	11.6 μg/mL Compound: docetaxel	Cytotoxicity against human KB cells by MTT assay Cytotoxicity against human KB cells by MTT assay	[PMID: 17624790]
KB 3-1	CC₅₀	0.25 nM Compound: Docetaxel	Cytotoxicity against human KB-3-1 cells after 72 hrs by MTS assay Cytotoxicity against human KB-3-1 cells after 72 hrs by MTS assay	[PMID: 18485536]
LLC-PK1	GI₅₀	0.17 nM Compound: Doc, Taxotere	Growth inhibition of pig LLC-PK1 cells by Alamar Blue assay Growth inhibition of pig LLC-PK1 cells by Alamar Blue assay	[PMID: 23956835]
LLC-PK1	GI₅₀	106.6 nM Compound: Doc, Taxotere	Growth inhibition of pig LLC-PK1 cells expressing MDR1 by Alamar Blue assay Growth inhibition of pig LLC-PK1 cells expressing MDR1 by Alamar Blue assay	[PMID: 23956835]
LoVo	IC₅₀	1.97 nM Compound: Taxotere	Cytotoxicity against human LoVo cells by SRB method Cytotoxicity against human LoVo cells by SRB method	[PMID: 18181575]
MCF7	IC₅₀	0.001 μM Compound: 1b	Concentration required for inhibition of 50% human breast MCF 7 cell line proliferation after 72 hr of incubation Concentration required for inhibition of 50% human breast MCF 7 cell line proliferation after 72 hr of incubation	[PMID: 15537348]
MCF7	IC₅₀	0.005 μM Compound: DOC	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 96 hrs by SRB assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 96 hrs by SRB assay	[PMID: 25817774]
MCF7	IC₅₀	1 nM Compound: Docetaxel	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 25047938]
MCF7	IC₅₀	1 nM Compound: docetaxel	Cytotoxicity against Pgp deficient human MCF7 cells after 72 hrs by sulforhodamine B test Cytotoxicity against Pgp deficient human MCF7 cells after 72 hrs by sulforhodamine B test	[PMID: 18465846]
MCF7	IC₅₀	1 nM Compound: docetaxel	Cytotoxicity against P-glycoprotein deficient wild type human MCF7 cells after 72 hrs Cytotoxicity against P-glycoprotein deficient wild type human MCF7 cells after 72 hrs	[PMID: 19239240]
MCF7	IC₅₀	1 nM Compound: 1b Docetaxel	Compound was tested for its cytotoxicity by inhibiting the growth of MCF-7 cell line after 72 h Compound was tested for its cytotoxicity by inhibiting the growth of MCF-7 cell line after 72 h	[PMID: 15225730]
MCF7	IC₅₀	1 nM Compound: Docetaxel	Inhibition of the growth against human mammary carcinoma (MCF-7) cell line after 72 hr exposure Inhibition of the growth against human mammary carcinoma (MCF-7) cell line after 72 hr exposure	[PMID: 9022794]
MCF7	IC₅₀	1 nM Compound: Docetaxel	In vitro cytotoxicity of compound against human MCF-7 (breast) cancer cell line after 72 hr of drug exposure In vitro cytotoxicity of compound against human MCF-7 (breast) cancer cell line after 72 hr of drug exposure	[PMID: 12477344]
MCF7	IC₅₀	1 nM Compound: Docetaxel	Inhibitory activity against the growth of MCF-7 (human breast carcinoma) cell line after 72 hours of drug exposure Inhibitory activity against the growth of MCF-7 (human breast carcinoma) cell line after 72 hours of drug exposure	[PMID: 10617084]
MCF7	IC₅₀	1 μM Compound: 2	inhibitory concentration against the growth of breast cancer MCF-7 cell line after 72 hr of exposure inhibitory concentration against the growth of breast cancer MCF-7 cell line after 72 hr of exposure	[PMID: 15501062]
MCF7	IC₅₀	11.9 μM Compound: docetaxel	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 22560315]
MCF7	IC₅₀	139.8 μM Compound: Docetaxel	Cytotoxicity against human MCF7 cells over-expressing CYP1B1 after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells over-expressing CYP1B1 after 48 hrs by MTT assay	[PMID: 25799264]
MCF7	IC₅₀	180 nM Compound: Docetaxel	Antiproliferative against multidrug-resistant human MCF7 cells after 72 hrs by MTT assay Antiproliferative against multidrug-resistant human MCF7 cells after 72 hrs by MTT assay	[PMID: 24835987]
MCF7	IC₅₀	215 nM Compound: docetaxel	Cytotoxicity against human MCF7 cells expressing P-glycoprotein after 72 hrs Cytotoxicity against human MCF7 cells expressing P-glycoprotein after 72 hrs	[PMID: 19239240]
MCF7	IC₅₀	235 nM Compound: Docetaxel	Inhibitory activity against the growth of MCF-7R ( multi drug resistant human breast carcinoma) cell line after 72 hours of drug exposure Inhibitory activity against the growth of MCF-7R ( multi drug resistant human breast carcinoma) cell line after 72 hours of drug exposure	[PMID: 10617084]
MCF7	IC₅₀	27.6 μM Compound: Docetaxel	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 25799264]
MCF7	IC₅₀	3.3 μg/mL Compound: docetaxel	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 17624790]
MCF7	IC₅₀	38.53 nM Compound: Docetaxel	Antiproliferative against human MCF7 cells after 72 hrs by MTT assay Antiproliferative against human MCF7 cells after 72 hrs by MTT assay	[PMID: 24835987]
MCF7	IC₅₀	4 μM Compound: docetaxel	Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 18226907]
MCF7R	IC₅₀	235 nM Compound: Docetaxel	Cytotoxicity against human MCF7/R cells by MTT assay Cytotoxicity against human MCF7/R cells by MTT assay	[PMID: 25047938]
MCF7R	IC₅₀	235 nM Compound: Docetaxel	Inhibition of the growth against human mammary carcinoma 180 fold resistant to doxorubicin (MCF-7-R) cell line after 72 hr exposure Inhibition of the growth against human mammary carcinoma 180 fold resistant to doxorubicin (MCF-7-R) cell line after 72 hr exposure	[PMID: 9022794]
MDA-MB-231	IC₅₀	0.018 nM Compound: Taxotere	Cytotoxicity against human MDA231 cells after 72 hrs by MTS assay Cytotoxicity against human MDA231 cells after 72 hrs by MTS assay	[PMID: 21142180]
MDA-MB-231	IC₅₀	1 nM Compound: DTX	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 24564494]
MDA-MB-435	IC₅₀	0.25 nM Compound: Taxotere	Cytotoxicity against human MDA435 cells after 72 hrs by MTS assay Cytotoxicity against human MDA435 cells after 72 hrs by MTS assay	[PMID: 21142180]
MHCC97H	IC₅₀	22.4 nM Compound: Docetaxel	Antiproliferative activity against human MHCC97H after 72 hrs by CellTiter Glo assay Antiproliferative activity against human MHCC97H after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
NCI/ADR-RES	GI₅₀	196 nM Compound: Doc, Taxotere	Growth inhibition of P-gp expressing human NCI-ADR-RES cells by Alamar Blue assay Growth inhibition of P-gp expressing human NCI-ADR-RES cells by Alamar Blue assay	[PMID: 23956835]
NCI/ADR-RES	IC₅₀	235 nM Compound: Docetaxel	In vitro cytotoxicity of compound against human MCF-7-MDR (breast) cancer cell line after 72 hr of drug exposure In vitro cytotoxicity of compound against human MCF-7-MDR (breast) cancer cell line after 72 hr of drug exposure	[PMID: 12477344]
NCI/ADR-RES	IC₅₀	5.18 μM Compound: DOC	Cytotoxicity against human MCF7/ADR cells assessed as growth inhibition after 96 hrs by SRB assay Cytotoxicity against human MCF7/ADR cells assessed as growth inhibition after 96 hrs by SRB assay	[PMID: 25817774]
NCI-H460	IC₅₀	0.0097 μM Compound: docetaxel	Cytotoxicity against human NCI-H460 cells after 72 hrs by SRB assay Cytotoxicity against human NCI-H460 cells after 72 hrs by SRB assay	[PMID: 19028425]
OVCAR-8	GI₅₀	0.5 nM Compound: Doc, Taxotere	Growth inhibition of human OVCAR8 cells by Alamar Blue assay Growth inhibition of human OVCAR8 cells by Alamar Blue assay	[PMID: 23956835]
P388	GI₅₀	1.62 ng/mL Compound: 2	Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against mouse leukemia P388 Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against mouse leukemia P388	10.1016/S0960-894X(97)10128-7
P388	GI₅₀	6.74 ng/mL Compound: 2 (Docetaxel)	Concentration required to inhibit the growth of mouse leukemia (P388) cell lines by 50% on 72 h continuous exposure Concentration required to inhibit the growth of mouse leukemia (P388) cell lines by 50% on 72 h continuous exposure	[PMID: 9871592]
PC-12	GI₅₀	19.1 ng/mL Compound: 2	Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against human lung cancer cell line PC-12 Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against human lung cancer cell line PC-12	10.1016/S0960-894X(97)10128-7
PC-12	GI₅₀	42.2 ng/mL Compound: 2 (Docetaxel)	Concentration required to obtain half of the maximal inhibition for cell growth in human non-small cell lung cancer cell line(PC-12) was determined by using MTT assay when the cells were exposed to compound for 72 hr Concentration required to obtain half of the maximal inhibition for cell growth in human non-small cell lung cancer cell line(PC-12) was determined by using MTT assay when the cells were exposed to compound for 72 hr	[PMID: 12270153]
PC-12	GI₅₀	53.4 ng/mL Compound: 2 (Docetaxel)	Concentration required to inhibit the growth of human lung cancer (PC-12)cell lines by 50% on 72 hr continuous exposure Concentration required to inhibit the growth of human lung cancer (PC-12)cell lines by 50% on 72 hr continuous exposure	[PMID: 9871592]
PC-3	IC₅₀	1.4 nM Compound: Taxotere	Cytotoxicity against human PC3 cells after 72 hrs by MTS assay Cytotoxicity against human PC3 cells after 72 hrs by MTS assay	[PMID: 21142180]
PC-3	GI₅₀	2.5 nM Compound: Doc, Taxotere	Growth inhibition of human PC3 cells after 72 hrs by Alamar Blue assay Growth inhibition of human PC3 cells after 72 hrs by Alamar Blue assay	[PMID: 23956835]
PC-3	GI₅₀	54 nM Compound: Doc, Taxotere	Growth inhibition of P-gp expressing docetaxel-resistant human PC3 cells after 72 hrs by Alamar Blue assay Growth inhibition of P-gp expressing docetaxel-resistant human PC3 cells after 72 hrs by Alamar Blue assay	[PMID: 23956835]
PC-3	IC₅₀	54.8 μM Compound: docetaxel	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 18226907]
PC-6	GI₅₀	1.13 ng/mL Compound: 2 (Docetaxel)	Concentration required to inhibit the growth of human lung cancer (PC-6)cell lines by 50% on 72 hr continuous exposure Concentration required to inhibit the growth of human lung cancer (PC-6)cell lines by 50% on 72 hr continuous exposure	[PMID: 9871592]
PC-6	GI₅₀	1.16 ng/mL Compound: 2	Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against human lung cancer cell line PC-6 Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against human lung cancer cell line PC-6	10.1016/S0960-894X(97)10128-7
PC-6	GI₅₀	1.48 ng/mL Compound: 2 (Docetaxel)	Concentration required to obtain half of the maximal inhibition for cell growth in human small cell lung cancer(PC-6) was determined by using MTT assay when the cells were exposed to compound for 72 hr Concentration required to obtain half of the maximal inhibition for cell growth in human small cell lung cancer(PC-6) was determined by using MTT assay when the cells were exposed to compound for 72 hr	[PMID: 12270153]
PC-6	GI₅₀	135 ng/mL Compound: 2 (Docetaxel)	Concentration required to obtain half of the maximal inhibition for cell growth in vincristine-resistant cell line expressing P-glycoprotein (PC-6/VCR29-9) was determined by using MTT assay when the cells were exposed to compound for 72 hr Concentration required to obtain half of the maximal inhibition for cell growth in vincristine-resistant cell line expressing P-glycoprotein (PC-6/VCR29-9) was determined by using MTT assay when the cells were exposed to compound for 72 hr	[PMID: 12270153]
PC-6	GI₅₀	173 ng/mL Compound: 2	Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against vincristine resistant cancer cell line PC-6/VCR Compound was tested for growth inhibitory activity after 72 hr of continuous exposure against vincristine resistant cancer cell line PC-6/VCR	10.1016/S0960-894X(97)10128-7
PLC-PRF-5	IC₅₀	16.7 nM Compound: Docetaxel	Antiproliferative activity against human PLC/PRF/5 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human PLC/PRF/5 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SGC-7901	IC₅₀	0.0084 μM Compound: docetaxel	Cytotoxicity against human SGC7901 cells after 72 hrs by SRB assay Cytotoxicity against human SGC7901 cells after 72 hrs by SRB assay	[PMID: 19028425]
SK-OV-3	IC₅₀	0.51 nM Compound: Taxotere	Cytotoxicity against human SKOV3 cells after 72 hrs by MTS assay Cytotoxicity against human SKOV3 cells after 72 hrs by MTS assay	[PMID: 21142180]
SK-OV-3	IC₅₀	3.4 nM Compound: Docetaxel	Cytotoxicity against human SKOV3 cells after 72 hrs by SRB assay Cytotoxicity against human SKOV3 cells after 72 hrs by SRB assay	[PMID: 18524425]
SK-OV-3	IC₅₀	5 nM Compound: 2, DTX, Taxotere	Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay	[PMID: 24269481]
SMMC-7721	IC₅₀	2 nM Compound: Docetaxel	Antiproliferative activity against human SMMC7721 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SMMC7721 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-182	IC₅₀	39.2 nM Compound: Docetaxel	Antiproliferative activity against human SNU182 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU182 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-387	IC₅₀	4.3 nM Compound: Docetaxel	Antiproliferative activity against human SNU387 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU387 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-398	IC₅₀	17.6 nM Compound: Docetaxel	Antiproliferative activity against human SNU398 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU398 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-423	IC₅₀	10.8 nM Compound: Docetaxel	Antiproliferative activity against human SNU423 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU423 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-449	IC₅₀	> 100 nM Compound: Docetaxel	Antiproliferative activity against human SNU449 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU449 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-475	IC₅₀	20.5 nM Compound: Docetaxel	Antiproliferative activity against human SNU475 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU475 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
U-87MG ATCC	IC₅₀	20.72 μg/mL Compound: Docetaxel	Cytotoxicity against human U87 cells after 72 hrs by MTT assay Cytotoxicity against human U87 cells after 72 hrs by MTT assay	10.1007/s00044-013-0582-8
WI-38	IC₅₀	> 10 μM Compound: docetaxel	Cytotoxicity against human WI38 cells after 72 hrs by SRB assay Cytotoxicity against human WI38 cells after 72 hrs by SRB assay	[PMID: 19028425]

In Vitro

Docetaxel Trihydrate (RP-56976 Trihydrate) and Glufosfamide (GLU) single and combined treatments affect the cells viability in a dose-dependent manner. The IC₅₀ of GLU are 70±4 μM and 86.8±8 μM in PC-3 and LNCaP cells; respectively. While, the IC₅₀ of Docetaxel alone is found to be 3.08±0.4 nM and 1.46±0.2 nM in PC-3 and LNCaP cells; respectively. The co-treatment of GLU with Docetaxel is found to synergize the cytotoxicity and the IC₅₀ values are decreased to be 2.7±0.1 nM and 0.75±0.3 nM in PC-3 and LNCaP cells; respectively^[1]. IC₅₀ of NCI-H460 to Docetaxel at 24 h is 116 nM and at 72 h is 30 nM. According to data reported in DTP Data Search, the mean IC₅₀ of NCI-60 cell panel to Docetaxel is 14-34 nM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (290.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 50 mg/mL (58.01 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1602 mL	5.8009 mL	11.6019 mL
5 mM	0.2320 mL	1.1602 mL	2.3204 mL
10 mM	0.1160 mL	0.5801 mL	1.1602 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 6

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Purity & Documentation

Purity: 99.92%

Data Sheet (292 KB) SDS (557 KB)

COA (267 KB) HNMR (337 KB) LCMS (257 KB)

Handling Instructions (2659 KB)

References

[1]. Attia RT, et al. The chemomodulatory effects of glufosfamide on docetaxel cytotoxicity in prostate cancer cells. PeerJ. 2016 Jun 29;4:e2168. [Content Brief]

[2]. Che CL, et al. DNA microarray reveals different pathways responding to paclitaxel and docetaxel in non-small cell lung cancer cell line. Int J Clin Exp Pathol. 2013 Jul 15;6(8):1538-48. [Content Brief]

[3]. Obi-Ioka Y, et al. Involvement of Wee1 in the circadian rhythm dependent intestinal damage induced by docetaxel. J Pharmacol Exp Ther. 2013 Oct;347(1):242-8. [Content Brief]

[4]. Li C, et al. Non-linear pharmacokinetics of piperine and its herb-drug interactions with docetaxel in Sprague-Dawley rats. J Pharm Biomed Anal. 2016 Sep 5;128:286-93. [Content Brief]

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