Dimethylsulfoxide (DMSO) for Cell Culture
Cat# D8050-01-100ml
Size : 100ml
Brand : US Biological
Grade
Cell Culture GradeShipping Temp
RTStorage Temp
RTMethyl Sulfoxide; Dimethylsulfoxide; DMSO
Ready-to-use sterile filtered product conveniently packaged in septum screw-capped amber bottles.
DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90% of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture of 10% DMSO, a freezing medium, and 30% fetal bovine serum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture) is used.
Synonyms:
Methyl Sulfoxide; Dimethylsulfoxide; DMSO
CAS No:
67-68-5
Molecular Formula:
C2H6SO
Molecular Weight:
78.13
Purity (GC):
≥99%
Specific Gravity:
1.095-1.097
Congealing Temp:
18.3°C
Refractive Index:
1.4755-1.4775
Water:
≤0.1%
Titratable Acid:
≤5ml
Residue after Evaporation:
≤0.01%
Absorbance:
To Pass ACS Specifications
Substances Darkened by KOH:
To Pass ACS Specifications
RNase:
None Detected
DNase:
None Detected
Endotoxin:
None Detected
Note: DMSO will congeal easily and may remain a solid at cool temperatures. Warm slightly to return to liquid state.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.