Specifications

Product Data
Clone Name	21H8
Applications	ELISA, IHC, ISH, WB
Recommended Dilution	ELISA: 1/1000-1/10000.  Southern Blot: 1/500-1/5000. Western Blot: > 1/500.  In Situ Hybridization. Immunohistochemistry: 1/200-1/2000.
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Digoxigenin
Specificity	Reacts with free and bound Digoxigenin.
Formulation	0.1M Tris, 0.1M Glycine, 2% Sucrose State: Purified State: Lyophilized powder Preservative: None
Reconstitution Method	Restore in distilled water
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Background	Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications.
Reference Data