DH10β Chemically Competent E. coli Cells
CAT#: CC100004
DH10β Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Specifications
Product Data | |
Genotype | F – mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara, leu)7697 galU galK rpsL (StrR) nupG λ- |
Components | DH10β Chemically Competent Cells – 1 mL (5 x 200 μL) Positive Control DNA - pUC19 (50pg/μl, 25 μl ) SOC medium – 20ml |
Features | Chemically Competent Accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA |
Applications | lacZ marker for blue/white color screening Transformation of cloned DNA into bacterial cells Ideal for subcloning and generating cDNA libraries |
Storage | The Competent Cells should be stored at -80°C. pUC19 Control DNA should be stored at -20°C and SOC recovery medium should be stored at 4°C immediately upon arrival. |
Stability | The cells are stable for 6 months from the date of shipment. |
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