DH10β Chemically Competent E. coli Cells

CAT#: CC100004

DH10β Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

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Specifications

Product Data
Genotype F – mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara, leu)7697 galU galK rpsL (StrR) nupG λ-
Components DH10β Chemically Competent Cells – 1 mL (5 x 200 μL)
Positive Control DNA - pUC19 (50pg/μl, 25 μl )
SOC medium – 20ml
Features Chemically Competent Accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA
Applications lacZ marker for blue/white color screening
Transformation of cloned DNA into bacterial cells
Ideal for subcloning and generating cDNA libraries
Storage The Competent Cells should be stored at -80°C. pUC19 Control DNA should be stored at -20°C and SOC recovery medium should be stored at 4°C immediately upon arrival.
Stability The cells are stable for 6 months from the date of shipment.

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