DEAE SepFast HighRes
Cat# 470501-1L
Size : 1litre
Brand : Biotoolomics
Contact local distributor :
Phone : +1 850 650 7790
Introduction
Q (DEAE, S, CM) SepFast™ HighRes is a group of ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.
The core advantages are:
- High resolution at high loading
- High sample loading capacity at high flow
- High productivity
The base matrix of IEX SepFast HighRes is made of highly cross-linked agarose. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
Characteristics of IEX SepFast HighRes
| Q SepFast HighRes | DEAE SepFast HighRes | S SepFast HighRes | CM SepFast HighRes | |
| Functionalgroup | Quaternary ammonium strong anion | Diethyl-aminoethyl weak anion | Sulfo strong cation | Carboxy- methyl weak cation |
| Total ionic capacity | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml |
| Particle size | 20 – 50 μm | |||
| Binding capacity* | >100 mg/ml BSA | >100 mg/ml BSA | >20 mg/ml hIgG | >100 mg/ml lysozyme |
| Pressure-flow property* | To be run at a residence time of 2 min and above. | |||
| Operational pressure | Up to 3 bar | |||
| pH stability | 2-14 (short term) and 3-12 (long term) | |||
| Working temperature | 4oC – 30oC | |||
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol | |||
| Total ionic capacity | Oxidizing agents, anionic detergents | Oxidizing agents, cationic detergents | ||
| Storage | 20% ethanol | 20% ethanol | 20% ethanol + 0.2M sodium acetate | 20% ethanol |
*Measured in a 32 mm ID column at a bed height of 15 cm.