Azb10021c | DANRE actba Antibody (Center) Biotrend

DANRE actba Antibody (Center) (Cat. #Azb10021c) western blot analysis in zebra fish muscle tissue lysates (35ug/lane).This demonstrates the DANRE actba antibody detected the DANRE actba protein (arrow).
All lanes : Anti-DANRE actba Antibody (Center) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, E
Primary Accession	Q7ZVI7
Other Accession	P60010, P68136, P68135, P68137, P68134, P68133, P68139, P68138, P63269, P63268, P63267, P63270, Q5E9B5, A2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P04751, P68035, P68033, P68032, P68034, Q3ZC07, O93400, P60711, P29751, Q6QAQ1, P60710, Q4R561, P60709
Reactivity	Zebrafish
Predicted	C.Elegans, Drosophila, Xenopus, Chicken, Bovine, Human, Mouse, Rabbit, Rat, Hamster, Horse, Monkey, Pig, Sheep, Yeast
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	41767 Da
Antigen Region	188-215 aa

Additional Information
Gene ID	57934
Other Names	Actin, cytoplasmic 1, Beta-actin-1, Actin, cytoplasmic 1, N-terminally processed, actba, bact, bactin1, bactzf
Target/Specificity	This DANRE actba antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of DANRE actba.
Dilution	WB~~1:1000
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DANRE actba Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	actba
Synonyms	bact, bactin1, bactzf
Function	Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
Cellular Location	Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:P60710}. Nucleus {ECO:0000250\|UniProtKB:O93400}
Tissue Location	Skeletal muscle, heart, gill, digestive tissue and brain (PubMed:9987040). Widespread expression throughout the brain, with highest levels in regions where neuronal proliferation is greatest (PubMed:9987040).

DANRE actba Antibody (Center)