Specifications

Product Data
Clone Name	UMAB249
Applications	IHC, WB
Recommended Dilution	IHC 1:200
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human CTLA4 (NP_001032720) produced in HEK293T cell.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage	Store at -20°C as received.
Stability	Stable for 12 months from date of receipt.
Predicted Protein Size	19.15 kDa
Gene Name	Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.
Database Link	NP_001032720 Entrez Gene 1493 Human UniProt ID P16410
Synonyms	ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Autoimmune thyroid disease, Cell adhesion molecules (CAMs), T cell receptor signaling pathway