Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Symbol	Corticotropin Releasing Factor Receptor 2
Synonyms	CRF-RB; CRF2; CRFR2; HM-CRF
Vector	PCMV6-Neo
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Restriction Sites	SgfI-MluI
ACCN	NM_001202475
Insert Size	1317 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001202475.1, NP_001189404.1
RefSeq Size	2904 bp
RefSeq ORF	1317 bp
Locus ID	1395
UniProt ID	Q13324
Cytogenetics	7p14.3
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
Gene Summary	The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011] Transcript Variant: This variant (2) contains additional in-frame coding exons at the 5' end compared to variant 1, resulting in an isoform (2, also known as beta isoform) with a longer and distinct N-terminus compared to isoform 1. This receptor subtype was shown to mediate urocortin-induced MAPK activation in human myometrium (PMID:11117536).