CNBr-activated SepFast MAG 4HF
Size : 1gram
Brand : Biotoolomics
CNBr activated SepFast MAG is magnetic agarose resin that can be readily employed to make various magnetic affinity chromatography adsorbents. The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized.
CNBr-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to CNBr-activated agarose is a widely used, successful, and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
CNBr activated SepFast MAG 4 High Flow (4HF) is made of highly cross-linked 4% agarose with magnetic properties.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The pre-activated base matrix can be readily employed to make various magnetic chromatography adsorbents for a variety of purification applications. The application areas cover immobilization of proteins, peptides and nucleic acids. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Coupling manual is supplied with each product.
Characteristics of CNBr-activated SepFast MAG 4HF
|Group to be coupled||-NH2|
|Coupling conditions||pH 6 - 10, 4oC to 30oC, 3-16 h, organic solvents possible|
|Matrix||Highly cross-linked 4% magnetic agarose|
|Particle size*||50 – 150 μm|
|Swelling factor||3 – 5 ml / g powder|
|Coupling level||>3 mg BSA / ml medium|
|pH stability||4 -11 (ligand dependent)|
|Chemical stability||Stable to all commonly used aqueous chemicals, provided the ligand to be coupled can withstand|
|Storage||4oC – 8oC|