Specifications

Product Data
Clone Name	PCG4.1
Applications	ELISA
Recommended Dilution	ELISA (1/1000).  Western Blot (Reported).
Reactivities	Clostridium difficile
Host	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Immunogen	C. difficile Toxin A. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Specificity	This antibody reacts with C. difficile Toxin A. Does not cross react with C. difficile Toxin B.
Formulation	10mM Sodium Phosphate, pH 7.4 ± 0.2 containing 150 mM Sodium Chloride State: Purified State: Liquid purified (0.2µm filtered) IgG fraction (>90% pure by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P16154
Background	Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhoea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.
Synonyms	toxA, tcdA
Note	Centrifuge before opening to ensure complete recovery of vial contents.
Reference Data