Chka (NM_013490) Mouse Tagged ORF Clone

CAT#: MR226317

  • TrueORF®

Chka (Myc-DDK-tagged) - Mouse choline kinase alpha (Chka), transcript variant 1

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK

Datasheet
SDS
Plasmid Map

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Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Chka
Synonyms Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha; EK; EtnK-alpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226317 representing NM_013490
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAACCAAGTTCTGCACCGGGGGCGAGGCCGAGCCGTCGCCTCTCGGGCTGCTGCTGAGCTGCGGTG
GCAACGCTGCCCCGACGCCCGGCGTAGGGCAGCAGCGGGACGCCGCAGGCGAGCTGGAGTCCAAGCAGCT
TGGTGGCCGAACCCAACCTCTCGCGCTGCCGCCGCCGCCGCCGCCGCCCCTGCCGCTGCCCCCGCCGCCA
TCACCGCCGCTAGCGGACGAACAACCCGAGCCCCGGACCAGGCGCAGGGCCTACCTGTGGTGTAAGGAAT
TCCTGCCCGGAGCCTGGCGAGGCCTTCGCGAGGACCAGTTCCACATCAGTGTCATCAGGGGTGGTCTCAG
TAACATGCTGTTCCAGTGCTCCCTGCCAGACTCCATAGCCAGCGTTGGCGATGAGCCTCGGAAAGTGCTC
TTGCGGCTCTATGGGGCAATCTTAAAGATGGGGGCTGAGGCGATGGTTCTGGAGAGTGTTATGTTTGCCA
TTCTTGCAGAGAGGTCACTTGGGCCAAAACTCTTTGGCATCTTTCCCCAAGGCCGACTGGAGCAGTTTAT
CCCGAGCCGGCGATTGGACACTGAAGAGTTACGTTTACCAGATATTTCTGCAGAAATAGCTGAGAAAATG
GCCACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAATGGCTTTTTGGAACAATGGAAAAAT
ACCTGAATCAAGTACTAAGACTCAAATTCAGCAGGGAGGCCAGAGTTCAGCAACTGCACAAGATCCTCTC
TTACAACCTGCCTCTTGAGCTCGAGAACCTGAGGTCATTGCTGCAGTATACTAGATCTCCAGTTGTATTT
TGTCATAATGACTGTCAAGAAGGTAATATCTTATTGTTGGAAGGCCAGGAGAACTCTGAAAGGCGGAAGC
TGATGCTTATTGACTTTGAGTACAGCAGTTACAATTACAGGGGATTTGACATTGGAAATCATTTCTGTGA
ATGGATGTATGATTATACCTATGAGAAGTATCCTTTCTTCAGAGCGAACATTCAGAAGTATCCTTCCCGA
AAACAACAGCTCCATTTTATTTCCAGTTACTTGACTACATTCCAAAATGATTTTGAAAGCCTCAGTAGTG
AAGAGCAGTTTGCTACAAAAGAAGACATGTTGCTTGAAGTCAACAGATTTGCTCTTGCCTCTCATTTCCT
CTGGGGACTTTGGTCCATTGTACAGGCCAAGATCTCATCCATTGAATTTGGGTACATGGAATATGCCCAA
GCCAGATTCGAGGCCTACTTTGACCAGAAGAGGAAGCTTGGGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR226317 representing NM_013490
Red=Cloning site Green=Tags(s)

MKTKFCTGGEAEPSPLGLLLSCGGNAAPTPGVGQQRDAAGELESKQLGGRTQPLALPPPPPPPLPLPPPP
SPPLADEQPEPRTRRRAYLWCKEFLPGAWRGLREDQFHISVIRGGLSNMLFQCSLPDSIASVGDEPRKVL
LRLYGAILKMGAEAMVLESVMFAILAERSLGPKLFGIFPQGRLEQFIPSRRLDTEELRLPDISAEIAEKM
ATFHGMKMPFNKEPKWLFGTMEKYLNQVLRLKFSREARVQQLHKILSYNLPLELENLRSLLQYTRSPVVF
CHNDCQEGNILLLEGQENSERRKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYTYEKYPFFRANIQKYPSR
KQQLHFISSYLTTFQNDFESLSSEEQFATKEDMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMEYAQ
ARFEAYFDQKRKLGV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013490
ORF Size 1305 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013490.4, NP_038518.2
RefSeq Size 2589 bp
RefSeq ORF 1308 bp
Locus ID 12660
UniProt ID O54804
Cytogenetics 19 3.57 cM
MW 50.4 kDa
Gene Summary Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions

SKU Description Size
MC215708 Chka (untagged) - Mouse choline kinase alpha (Chka), transcript variant 1, (10ug)
    • 10 ug

503,00 EUR

MG226317 Chka (tGFP-tagged) - Mouse choline kinase alpha (Chka) transcript variant 1, (10ug)
    • 10 ug

657,00 EUR

MR226317L3 Lenti ORF clone of Chka (Myc-DDK-tagged) - Mouse choline kinase alpha (Chka), transcript variant 1
    • 10 ug

757,00 EUR

MR226317L4 Lenti ORF clone of Chka (mGFP-tagged) - Mouse choline kinase alpha (Chka), transcript variant 1
    • 10 ug

757,00 EUR