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> CHAPS (hydrate) [331717-45-4]

CHAPS (hydrate) [331717-45-4]

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Cat# HY-15435A-1g

Size : 1g

Brand : MedChemExpress

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Phone : +1 850 650 7790

CHAPS hydrate, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS hydrate is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution.

For research use only. We do not sell to patients.

CHAPS hydrate Chemical Structure

CHAPS hydrate Chemical Structure

CAS No. : 331717-45-4

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of CHAPS hydrate:

  • CHAPS In-stock
Description

CHAPS hydrate, a derivative of Cholic acid, is a zwitterionic detergent for solubilizing membrane proteins. CHAPS hydrate is used for stabilization of various protein-DNA complexes and can retain biochemical activity of proteins in solution[1][2].

Molecular Weight

632.89

Formula

C32H60N2O8S
CAS No.

331717-45-4
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@]2(18)[C@@]3(18)[C@@]([C@@](CC3)(18)[C@H](C)CCC(NCCC[N+](C)(C)CCCS(=O)([O-])=O)=O)([C@H](C[C@]2(18)[C@@]4([C@](C[C@@H](CC4)O)(18)C1)C)O)C.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 10.87 mg/mL (17.18 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5801 mL 7.9003 mL 15.8005 mL
5 mM 0.3160 mL 1.5801 mL 3.1601 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

  • [1]. Kroflic A, et al. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71.  [Content Brief]

    [2]. Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137.  [Content Brief]

  • [1]. Kroflic A, et al. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71.

    [2]. Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5801 mL 7.9003 mL 15.8005 mL 39.5013 mL
5 mM 0.3160 mL 1.5801 mL 3.1601 mL 7.9003 mL
10 mM 0.1580 mL 0.7900 mL 1.5801 mL 3.9501 mL
15 mM 0.1053 mL 0.5267 mL 1.0534 mL 2.6334 mL
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CHAPS hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CHAPS331717-45-4Biochemical Assay ReagentszwitterionicdetergentsurfactantproteincomplexesstabilizedInhibitorinhibitorinhibit