Reagents and instruments for immunology, cell biology and molecular biology.
 
> CF®405M Succinimidyl Ester

CF®405M Succinimidyl Ester

Print Print
Please log in

Cat# 92111

Size : 1UMOL

Brand : Biotium

Request more information

Contact local distributor :


Phone : +1 850 650 7790
CF®405M Succinimidyl Ester
CF® Dye Succinimidyl Ester
Chemical reactivity (reacts with)

Amines
Functional group

Tetrafluorophenyl Ester (TFP Ester), Succinimidyl Ester (NHS Ester)
Storage Conditions

Store at -10 to -35 °C, Protect from light
Protocols
SDS
Supporting Documents
Download Datasheet

Product Description

CF® Dye SE/TFP Esters are amine-reactive forms of Biotium’s bright and photostable CF® Dyes. The succinimidyl ester (SE) group, also known as N-hydroxysuccinimide (NHS) esters, on the dye molecule reacts with primary amines such as lysine residues of antibodies or proteins, amine-modified oligonucleotides and other amine-containing molecules to form a stable amide linkage. CF® Dye TFP (tetrafluorophenyl) esters are more stable alternatives to succinimidyl ester. CF® Dye TFP esters are available for CF®850 and CF®870, Biotium’s industry-leading near-infrared dyes with emission above 850 nm.

  • Amine-reactive fluorescent dyes with high labeling efficiency
  • Label antibodies, proteins, amine-modified oligonucleotides and other amine-containing molecules.
  • Offered with Biotium’s bright and photostable CF® Dyes which provide superior signal-to-noise
  • Available in over 40 CF® Dye colors from blue to near-IR wavelengths
  • Near-IR CF®850 and CF®870 TFP esters offer emission above 850 nm

Due to Biotium’s unique manufacturing and packaging process, our amine-reactive CF® Dyes are provided with the highest purity and more stable ester dye forms than other vendors. As a result, our CF® Dye SE/TFP products retain more reactivity and provide a higher labeling efficiency for users.

Superior CF® Dyes

CF® Dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability, and water solubility compared to other commercially available fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.

Also see our CF® Dye SE Protein Labeling Kits and CF® Dye Mix-n-Stain™Antibody Labeling Kits.

CF® Dye SE/TFP Ester

Dye Ex/Em Size Catalog No. Dye Features
CF®350 SE 347/448 nm 1 umol 92109 CF®350 Features
CF®405S SE 404/431 nm 1 umol 92110 CF®405S Features
CF®405M SE 408/452 nm 1 umol 92111 CF®405M Features
CF®405L SE 395/545 nm 1 umol 92112 CF®405L Features
CF®410 SE 404/455 nm 5 mg 97502
CF®430 SE 426/498 nm 1 umol 92117 CF®430 Features
CF®440 SE 440/515 nm 1 umol 92123 CF®440 Features
CF®450 SE 450/538 nm 1 umol 96011 CF®450 Features
CF®488A SE 490/515 nm 1 umol 92120 CF®488A Features
CF®503R SE 503/532 nm 1 umol 96078 CF®503R Features
CF®505 SE 505/519 nm 1 umol 97500 Structurally equivalent to ATTO 488
CF®514 SE 516/548 nm 1 umol 92103 CF®514 Features
CF®532 SE 527/558 nm 1 umol 92104 CF®532 Features
CF®543 SE 541/560 nm 1 umol 92105 CF®543 Features
CF®550R SE 551/577 nm 1 umol 96073 CF®550R Features
CF®555 SE 555/565 nm 1 umol 92130 CF®555 Features
CF®568 SE 562/583 nm 1 umol 92131 CF®568 Features
CF®570 SE 568/591 nm 1 umol 96014 CF®570 Features
CF®583 SE 583/606 nm 1 umol 96016 CF®583 Features
CF®583R SE 586/609 nm 1 umol 96084 CF®583R Features
CF®594 SE 593/614 nm 1 umol 92132 CF®594 Features
CF®597R SE 597/619 nm 1 umol 96092 CF®597R Features
CF®620R SE 617/639 nm 1 umol 92106 CF®620R Features
CF®633 SE 630/650 nm 1 umol 92133 CF®633 Features
CF®640R SE 642/662 nm 1 umol 92108 CF®640R Features
CF®647 SE 650/665 nm 1 umol 92135 CF®647 Features
CF®660R SE 663/682 nm 1 umol 92134 CF®660R Features
CF®660C SE 667/685 nm 1 umol 92137 CF®660C Features
CF®680 SE 681/698 nm 1 umol 92139 CF®680 Features
CF®680R SE 680/701 nm 1 umol 92107 CF®680R Features
CF®700 SE 695/720 nm 1 umol 96067 CF®700 Features
CF®710 SE 712/736 nm 1 umol 96125 CF®710 Features
CF®725 SE 729/750 nm 1 umol 96126 CF®725 Features
CF®740 SE 742/767 nm 1 umol 96109 CF®740 Features
CF®750 SE 755/779 nm 1 umol 92142 CF®750 Features
CF®770 SE 770/797 nm 1 umol 92150 CF®770 Features
CF®790 SE 784/806 nm 0.25 umol 92155 CF®790 Features
CF®800 SE 797/816 nm 0.25 umol 92127 CF®800 Features
CF®820 SE 822/835 nm 0.25 umol 96068 CF®820 Features
CF®850 TFP 852/870 nm 0.25 umol 96094 CF®850 Features
CF®870 TFP 876/896 nm 0.25 umol 96095 CF®870 Features

CF® Dye Technical Data

Dye Abs/Em (nm) MW (free acid form) Sephadex® media1 Amax (nm) Cf A260/Amax Cf A280/Amax ε2 Optimal DOL (IgG)
CF®350 347/448 ~496 G-25 347 0.13 0.14 18,000 4-6
CF®405S 404/431 ~1169 G-25 404 0.19 0.7 33,000 5-10
CF®405M 408/452 ~503 G-25 408 0.24 0.13 41,000 4-6
CF®405L 395/545 ~1573 G-25 395 N/A 0.5 24,000 8-12
CF®410 404/455 ~242 G-25 416 0.15 0.2 46,000 5-7
CF®430 426/498 ~429 G-25 426 0.21 0.044 40,000 5-8
CF®440 440/515 ~479 G-25 440 0.303 0.139 40,000 5-8
CF®450 405/460 ~689 G-25 450 0.205 0.2 40,000 5-8
CF®488A 490/515 ~914 G-25 490 0.16 0.1 70,000 7-9
CF®503R 503/532 ~1100 G-25 503 0.21 0.09 90,000 4-10
CF®505 505/519 ~587 G-25 505 0.22 0.09 90,000 4-8
CF®514 516/548 ~1216 G-25 516 0.14 0.073 105,000 5-8
CF®532 527/558 ~685 G-25 527 0.11 0.06 96,000 4-7
CF®543 541/560 ~887 G-25 541 0.305 0.095 100,000 4-7
CF®550R 551/577 ~686 G-25 551 0.12 0.08 100,000 5-6
CF®555 555/565 ~959 G-25 555 0.026 0.08 150,000 4-5, 3-6*
CF®568 562/583 ~714 G-25 562 0.17 0.08 100,000 5-6
CF®570 568/591 ~2998 G-25 568 0.0998 0.1 150,000 5-6
CF®583 583/606 ~3127 G-75 583 0.139 0.223 150,000 5-6
CF®583R 585/609 ~773 G-25 585 0.19 0.08 100,000 5-6
CF®594 593/614 ~729 G-25 593 0.24 0.08 115,000 4-7
CF®597R 597/619 ~800 G-25 597 0.25 0.08 100,000 5-6
CF®620R 617/639 ~738 G-25 617 0.28 0.45 115,000 5-6
CF®633 630/650 ~821 G-25 630 0.25 0.48 100,000 4-7
CF®640R 642/662 ~832 G-50 642 0.23 0.44 105,000 4-7
CF®647 650/665 ~985 G-25 650 0.01 0.03 240,000 4-5, 3-6*
CF®660C 667/685 ~3024 G-75 667 0.05 0.08 200,000 3-6, 2-3*
CF®660R 663/682 ~888 G-25 663 0.2 0.51 100,000 4-7
CF®680 681/698 ~3153 G-75 681 0.06 0.09 210,000 3-5, 2-3*
CF®680R 680/701 ~912 G-25 680 0.155 0.32 140,000 5-6
CF®700 696/721 ~2474 G-75 696 0.055 0.06 240,000 3-6
CF®710 712/736 ~860 G-25 712 0.11 0.07 115,000 5-6
CF®725 729/750 ~890 G-25 729 0.11 0.07 120,000 5-6
CF®740 742/767 ~900 G-25 742 0.132 0.08 105,000 5-6
CF®750 755/777 ~2921 G-75 755 0.01 0.03 250,000 3-5, 2-3*
CF®770 770/797 ~3091 G-75 770 0.041 0.06 220,000 3-5, 2-3*
CF®790 784/806 ~3179 G-75 784 0.104 0.07 210,000 3-5
CF®800 797/816 ~3334 G-75 797 0.09 0.08 210,000 3-5
CF®820 822/835 ~2711 G-75 822 0.0459 0.07 253,000 3-6
CF®850 852/870 ~2787 G-75 852 N/A 0.06 240,000 3-6
CF®870 876/896 ~2773 G-75 877 N/A 0.06 240,000 3-6
1. Sephadex® media recommendations are for antibody purification, not nucleic acid.
2. Extinction Coefficient (ε).
*Suitable, but suboptimal DOL.

Sephadex is a registered trademark of Cytiva.

References

1. Biomater. Sci.(2019) 7, 1898-1904. DOI:10.1039/C8BM01378K
2. Biomaterials (2015) 67, 382-392. DOI:10.1016/j.biomaterials.2015.07.046
3. Microbiological Research (2018) 217, 69-80. DOI:10.1016/j.micres.2018.08.017

Download a list of CF® Dye references.

Powered by Bioz

See more details on Bioz

 

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT
92112
CF®405L Succinimidyl Ester
 1UMOL 
92110
CF®405S Succinimidyl Ester
 1UMOL