CERK1 - Chitin elicitor receptor kinase 1
Cat# AS164037
Size : 50µg
Brand : Agrisera
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Product no: AS16 4037
AS16 4037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
319 €
Delivery: 3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RPS4 sequence, UniProt: A8R7E6, TAIR: At3g21630
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1: 1000 - 1: 3500 (WB)
Expected | apparent MW: 67 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana
Predicted reactivity: Arabidopsis thaliana Not reactive in: Nicotiana benthamiana, Solanum lycopersicum
- Application Examples
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Application example
Total protein from 16-day old Arabidopsis thaliana Col-0 (wild-type) and cerk1-2 null mutant (Cao et al., 2014). Total protein was extracted using Buffer H (Heese et al., 2007; LaMontagne et al., 2016) except that 40 nM Calyculin was used. 70 µg of total protein was denatured at 65°C for 5 min, separated on an 8% SDS-PAGE and transferred for 1h using a tank transfer system to nitrocellulose membrane. Blots were blocked with 1x PBS (Fisher Scientific BP665-1) + 0.1 %Tween 20 (PBS-T) + 5% milk for 1.5h at room temperature (RT) with agitation. Blots were incubated with primary antibody at a dilution of 1: 1000 overnight at 4°C with agitation in 1x PBS-T + 5% milk. The antibody solution was decanted, and the blot washed four times (1x 5min and 3x 6min) in 1x PBS-T at RT with agitation. The blot was then incubated with secondary antibody (Goat anti Rabbit IgG (H&L)–HRP from Agrisera (AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
213 €AS10 687 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. bentamiana, S. oleracea, S. lycopersicum, T. aestivum, Z. mays, V. vinifera
357 €Buy 2 items of this product for 226.00 €/itemsBuy 3 items of this product for 205.00 €/itemsAS12 1854 Clonality: Polyclonal Host: Rabbit | Reactivity: Arabidopsis thaliana
319 €Buy 2 items of this product for 239.00 €/itemsBuy 3 items of this product for 217.00 €/itemsLogin / Retailer