Specifications

Product Data
Clone Name	MEM-259
Applications	FC, IF
Recommended Dilution	Flow Cytometry: analysis of Human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension.  The content of a vial (2 ml) is sufficient for 100 tests.
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	HPB-ALL T cell line
Specificity	This antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Formulation	Phosphate Buffered Saline (PBS) Label: FITC State: Liquid purified Ig fraction Preservative: 15 mM Sodium Azide Label: Fluorescein Isothiocyanate. The reagent is free of unconjugated and adjusted for direct use
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C.  Do Not Freeze!  This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 967 Human UniProt ID P08962
Background	CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Synonyms	OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491
Reference Data