CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: Psel.KO.2.12]

Specifications

Product Data
Clone Name	Psel.KO.2.12
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat CD62P antibody to label 106 cells in 100 µl.
Reactivities	Goat, Human, Rat, Sheep
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	CD62P transfected 300.19 cells. Spleen cells from immunised CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Specificity	This antibody recognises the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. Clone Psel.KO.2.12 is reported to inhibit P-selectin-dependent adhesion between HL60 cells and P-selectin transfected COS cells. We recommend the use of Azide Free, Low Endotoxin CD62P antibody Cat.-No SM2262LE for this purpose.
Formulation	PBS Label: PE State: Lyophilised purified IgG fraction Stabilizer: 1% BSA and 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1.0 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	PE
Storage	Store undiluted at 2-8°C Prior to and following reconstitution . DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Gene Name	selectin P
Database Link	Entrez Gene 6403 Human UniProt ID P16109
Background	CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.
Synonyms	SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3
Reference Data