CD45 (CD45R) Mouse Monoclonal Antibody [Clone ID: 20.96]

Specifications

Product Data
Clone Name	20.96
Applications	FC
Recommended Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 1 x 106 cells in 100 µl.
Reactivities	Sheep
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Ovine thymocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Specificity	This antibody recognises the CD45R cell surface antigen.
Formulation	PBS, pH 7.4 Label: PE State: Lyophilized purified IgG fraction from tissue culture supernatant Stabilizer: 1% BSA, 5% Sucrose Preservative: 0.09% Sodium Azide Label: R. Phycoerythrin (RPE)
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	Leukocyte common antigen, L-CA, PTPRC, T200, CD45R
Reference Data