Specifications

Product Data
Clone Name	MEM-158
Applications	FC
Recommended Dilution	Suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivities	Human
Host	Mouse
Isotype	IgM
Clonality	Monoclonal
Immunogen	Human granulocytes.
Specificity	The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. Negative Species: Porcine.
Formulation	PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer. Label: PE State: Liquid purified IgG fraction. Label: Conjugated with R-Phycoerythrin under optimum conditions
Purification	Size-exclusion chromatography.
Conjugation	PE
Storage	Store the antibody in the dark at 2-8°C. Do Not Freeze! Avoid prolonged exposure to light.
Stability	Shelf life: one year from despatch.
Background	CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Synonyms	Lewis X, X-Hapten, Lacto-N-Fucopentaose III, Stage-Specific Embryonic Antigen, SSEA1
Reference Data