CCL2 Chemi-Luminescent ELISA Kit (Dog)
Cat# OKCD03740
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for CCL2 Chemi-Luminescent ELISA Kit (Dog) (OKCD03740) |
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| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
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| Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Monocyte Chemotactic Protein 1 (MCP1) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 0.41-300ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.41 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | CCL2 |
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| Gene Full Name | C-C motif chemokine ligand 2 |
| Alias Symbols | C-C motif chemokine 2;chemokine (C-C motif) ligand 2;MCP-1;Monocyte chemoattractant protein 1;monocyte chemoattractant protein-1;monocyte chemotactic protein 1;small-inducible cytokine A2. |
| NCBI Gene Id | 403981 |
| Protein Name | C-C motif chemokine 2 |
| Description of Target | Chemotactic factor that attracts monocytes, but not neutrophils. Important factor in the course of the inflammatory reaction to reperfusion of the previously ischemic myocardium. May play a significant role in monocyte trafficking into the reperfused myocardium. |
| Uniprot ID | P52203 |
| Protein Accession # | NP_001003297 |
| Nucleotide Accession # | NM_001003297 |