Catalase Fluorescent Activity Kit
Cat# OKAU00030
Size : 2plate
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Catalase Fluorescent Activity Kit (OKAU00030) |
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| Application | AA | ||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Fluorometric | ||||||||||||||||||||||||||||||
| ELISA Kit Linearity | Linearity was determined by taking two samples, one with a high known catalase activity of 3.74 U/mL and the other with a lower catalase activity of 0.90 U/mL and mixing them in the ratios given below. The measuredactivities were compared to the expected values based on the ratios used.
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| ELISA Kit Principle | The DetectX® Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. Please read the complete kit insert before performing this assay. A bovine catalase standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area black plate. Hydrogen peroxide is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Fluorescent Detection Reagent is added, followed by diluted horseradish peroxidase and incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a fluorescent product. The fluorescent product is read at 590 nm with excitation at 570 nm. Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in fluorescent product. The activity of the catalase in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of catalase activity per mL. | ||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra Assay Precision Three samples diluted in Assay Buffer were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were:
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| Additional Information | Background: Hydrogen peroxide, H2O2 is one of the most frequently occurring reactive oxygen species. It is formed either in the environment or as a by-product of aerobic metabolism, superoxide formation and dismutation, or as a product of oxidase activity. Both excessive hydrogen peroxide and its decomposition product hydroxyl radical, formed in a Fenton-type reaction, are harmful for most cell components. Its rapid removal is essential for all aerobically living prokaryotic and eukaryotic cells. Hydrogen peroxide however can act as a second messenger in signal transduction pathways, in immune cell activation, inflammation processes, cell proliferation, and apoptosis. Catalase Catalase Reaction 2 H2 O2 => H2 O + O2 One of the most efficient ways of removing peroxide is through the enzyme catalase, which is encoded by a single gene, and is highly conserved among species. Mammals, including humans and mice, express catalase in all tissues, and a high concentration of catalase can be found in the liver, kidneys and erythrocytes. The expression is regulated at transcription, post-transcription and post-translation levels. High catalase activity is detected in peroxisomes. More recently, short wavelength UV radiation has been shown to produce reactive oxygen species (ROS) through the action of catalase. This response is thought to act as a mechanism to protect DNA by converting damaging UV radiation into ROS species that can be metabolized and detoxified by cellular antioxidant enzymes. | ||||||||||||||||||||||||||||||
| :: | Detection Limit: Limit of Detection was determined as 0.112 U/mL. This is equivalent to 2.80 mU/well. | ||||||||||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cells, Tissues and Erythrocyte Lysates | ||||||||||||||||||||||||||||||
| Sensitivity | Sensitivity was determined as 0.073 U/mL. This is equivalent to 1.83 mU/well. |
| Gene Full Name | Catalase |
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