Bovine Serum Albumin (BSA)(ALB/398), Horseradish Peroxidase conjugate, 0.1mg/mL

Cat# BNCH0398-100

Size : 100uL

Brand : Biotium

Contact local distributor :


Phone : +1 850 650 7790

Bovine Serum Albumin Monoclonal Mouse Antibody (ALB/398)

Product Attributes

Antibody number

#0398

Antibody reactivity (target)

Bovine Serum Albumin

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

ALB/398

Isotype

IgG1

Molecular weight

66 kDa

Synonyms

ALB; BSA; Growth inhibiting protein 20

Entrez gene ID

280717 (Cow)

SwissProt

P02769 (Cow)

Unigene

Bt.106669 (Cow)

Immunogen

Purified bovine serum albumin

Antibody target cellular localization

Secreted (extracellular), Vesicular

Species reactivity

Cow

Antibody application notes

For coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry, Does not react with human, rabbit, dog, or guinea pig, others not tested

Positive control

Bovine serum albumin. Cow liver

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Product Description

This antibody reacts with a protein of ~66 kDa, identified as bovine serum albumin (BSA). It is a high affinity antibody and can be used for detection of traces of BSA. BSA is an abundant plasma protein in cows that is important for maintaining osmotic pressure in blood plasma for proper distribution of body fluids between intravascular compartments and body tissues. BSA is a common buffer component in immunoglobulin type assays due to good solubility characteristics for water, calcium, sodium, potassium, fatty acids, hormones and bilirubin. BSA makes up about half of the protein in plasma and represents the most stable and soluble protein in the plasma. It is a suitable reagent for laboratories developing immunoassays, mostly due to its availability, solubility and the numerous functional groups present for coupling. BSA contains several lysines that are capable of reacting with conjugation sites of linkers, making it applicable as a carrier protein for antigenic compounds.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@biotrend.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 0398, Anti-Bovine Serum Albumin (ALB/398)

Antibody # prefix Conjugation Ex/Em (nm) Compatible Lasers (nm) Fluorescence detection channels Supplied as Special applications/notes
BNC04 CF®405S 404/431 405 DAPI (microscopy), Alexa Fluor® 405 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide SIM
BNC05 CF®405M 408/452 405 DAPI (microscopy), Pacific Blue™ 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide STORM, STED, SIM, 2-Photon, TIRF
BNC88 CF®488A 490/515 488 GFP, FITC 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide STORM, STED, 2-photon, TIRF
BNC43 CF®543 541/560 532 RFP, TRITC 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNC55 CF®555 555/565 532, 561 RFP, TRITC 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide STORM
BNC68 CF®568 562/583 532, 561 RFP, TRITC 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide STORM, STED, SIM, TIRF
BNC94 CF®594 593/614 561 Texas Red® 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide STORM, STED, 2-photon
BNC40 CF®640R 642/662 633-640 Cy®5 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide SIM, TIRF, and FLImP
BNC47 CF®647 650/665 633-640 Cy®5 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide STORM
BNC61 CF®660R 663/682 633-640 Cy®5 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide Highly photostable
BNC80 CF®680 681/698 633-640 Cy®5.5, IR®700 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide STORM, 3D SMLM, LI-COR® Odssey®
BNC81 CF®680R 680/701 633-640 Cy®5.5, IR®700 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide 2-photon, STED, SMT, LI-COR® Odyssey®; Highly photostable
BNC70 CF®770 770/797 785, 808 IR®800 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide LI-COR® Odyssey®
BNCR R-PE (PE) 496, 546, 565/578 488-561 PE 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCA APC 650/660 594-633 APC 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCP PerCP 482/677 488-561 PerCP, PE-Cy®5 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCB Biotin N/A N/A N/A 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCAP Alkaline Phosphatase N/A N/A N/A 0.1 mg/mL PBS, 0.1% BSA, 0.05% azide
BNCH Horseradish Peroxidase N/A N/A N/A 0.1 mg/mL PBS, 0.05% BSA, no azide
BNUB Purified, with BSA N/A N/A N/A 0.2 mg/mL PBS, 0.05% BSA, 0.05% azide
BNUM Purified, BSA-free N/A N/A N/A 1 mg/mL PBS, no BSA, no azide
The prefix indicates conjugation, followed by the antibody number and size suffix.

Alexa Fluor, Pacific Blue and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.