Detection of human IgG1 in monkey serum, using ELISA showing RM129 reacts only to kappa and ELISA showing RM129 does not react to monkey A titer Sandwich ELISA using Biotinylated RM129
Product Details Product Name Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129 Product Description Biotinylated Rabbit monoclonal to Human Immunoglobulin Light Chains Catalog No. 32-1031-02 Clone Name RM129 Product Data Sheet Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody Data Sheet RM129 Specificity RM129 reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. Immunogen Human IgG Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Species Reactivity Human Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.02 µg/mL – 0.25 µg/mL;
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Flow Cytometry (FC): 0.5 µg/mL - 2 µg/mL. Keywords . Product Images
RM117 (capture) and biotin-RM129 (detection)
as a Sandwich ELISA pair. HRP conjugated
streptavidin and TMB were used to yield the
colorimetric reaction.
lambda light chain of all human immunoglobulins,
not to mouse IgG, rat IgG, or goat IgG.
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM129 and a monkey IgG binding
antibody (positive control) was used as the detection
antibody.
as the detection antibody. The plate, coated
with the capture antibody anti-human IgG RM116,
was loaded with different amounts of human IgG.
A serial dilution of Biotin RM129 was used as
the detection antibody, followed by an alkaline
phosphatase conjugated streptavidin.
Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129 PBS only
Cat# 32-1031-02
Size : 1mg
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Biotinylated Rabbit monoclonal to Human Immunoglobulin Light Chains
50 µg
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