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> Anti-XRCC1 (CHICKEN) Antibody

Anti-XRCC1 (CHICKEN) Antibody

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Cat# 200-901-MG5

Size : 100ug

Brand : Rockland Immunochemicals

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Phone : +1 850 650 7790

Background

XRCC1 (X-Ray Repair Cross Complementing 1) is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Diseases associated with XRCC1 include Spinocerebellar Ataxia, Autosomal Recessive 26 and Gastric Cardia Carcinoma. Anti-XRCC1 Antibody is useful for researchers interested in Cancer research and DNA repair.

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Specifications for XRCC1 Antibody

Product Details

Anti-XRCC1 (CHICKEN) Antibody - 200-901-MG5
Chicken Anti-X-Ray Repair Cross Complementing 1 Antibody, X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 1, X-Ray Repair Cross-Complementing Protein 1, DNA Repair Protein XRCC1, SCAR26, RCC
Chicken
Polyclonal
IgY

Target Details

XRCC1  - View All XRCC1 Products
Human, Mouse
Conjugated Peptide
Anti-XRCC1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-Terminal portion of human XRCC1 conjugated to Keyhole Limpet Hemocyanin (KLH). A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human and 91% to mouse, rat, and Chinese hamster.
This purified antibody is directed against human XRCC1.  This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. 
UniProtKB - P18887
NCBI - NP_006288.2
GeneID - 7515

Application Details

IF, WB
Anti-XRCC1 Antibody has been tested in immunofluorescence and western blot.  Expect a band at ~69.5kDa in western blot using appropriate tissues and lysates.  Positive control used: U2OS, MOLT4, Jurkat, and NIH-3T3 in WB.  U2OS in Immunofluorescence.

Formulation

Liquid (sterile filtered)
0.93 mg/ml by UV absorbance at 280 nm
0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol
Fluorescent Western Blotting Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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