Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-SMC4 antibody [S7MR]

Anti-SMC4 antibody [S7MR]

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Cat# NB-22-48316-100

Size : 100μL

Brand : Neo Biotech

Contact local distributor :


Phone : +1 850 650 7790

  • Western blot analysis of extracts of various cell lines, using SMC4 rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of C6 cells using SMC4 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using SMC4 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using SMC4 rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

General Info

Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SMC4 (1189-1288) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol: SMC4
Gene ID: 10051
Uniprot ID: SMC4_HUMAN
Immunogen Region: 1189-1288
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1189-1288 of human SMC4 (Q9NTJ3).
Immunogen Sequence: FNLSGGEKTLSSLALVFALH HYKPTPLYFMDEIDAALDFK NVSIVAFYIYEQTKNAQFII ISLRNNMFEISDRLIGIYKT YNITKSVAVNPKEIASKGLC

Description

Tissue Specificity Widely expressed. Higher expression in testis, colon, thymus.
Function Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
Protein Name Structural Maintenance Of Chromosomes Protein 4
Smc Protein 4
Smc-4
Chromosome-Associated Polypeptide C
Hcap-C
Xcap-C Homolog
Database Links Reactome: R-HSA-2299718
Reactome: R-HSA-2514853
Cellular Localisation Nucleus
Cytoplasm
Chromosome
In Interphase Cells
The Majority Of The Condensin Complex Is Found In The Cytoplasm
While A Minority Of The Complex Is Associated With Chromatin
A Subpopulation Of The Complex However Remains Associated With Chromosome Foci In Interphase Cells
During Mitosis
Most Of The Condensin Complex Is Associated With The Chromatin
At The Onset Of Prophase
The Regulatory Subunits Of The Complex Are Phosphorylated By Cdc2
Leading To Condensin's Association With Chromosome Arms And To Chromosome Condensation
Dissociation From Chromosomes Is Observed In Late Telophase
Alternative Antibody Names Anti-Structural Maintenance Of Chromosomes Protein 4 antibody
Anti-Smc Protein 4 antibody
Anti-Smc-4 antibody
Anti-Chromosome-Associated Polypeptide C antibody
Anti-Hcap-C antibody
Anti-Xcap-C Homolog antibody
Anti-SMC4 antibody
Anti-CAPC antibody
Anti-SMC4L1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance