Anti-SARS-CoV-2-NSP13 antibody

Cat# NB-22-72474-200

Size : 200μL

Brand : Neo Biotech

Contact local distributor :


Phone : +1 850 650 7790

  • Western blot analysis of extracts of normal 293T cells and 293T transfected with NSP13 Protein, using SARS-CoV-2 NSP13 antibody at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 NSP13 (His-tag) and untreated 293T cells using SARS-CoV-2 NSP13 rabbit polyclonal antibody at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.

General Info

Host: Rabbit
Applications: WB/IF
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2-NSP13 (1-601) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen Region: 1-601
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-601 of coronavirus NSP13 (YP_009725308.1).
Immunogen Sequence: AVGACVLCNSQTSLRCGACI RRPFLCCKCCYDHVISTSHK LVLSVNPYVCNAPGCDVTDV TQLYLGGMSYYCKSHKPPIS FPLCANGQVFGLYKNTCVGS DNVTDFNAIATCDWTNAGDY ILANTCTERLKLFAAETLKA TEETFKLSYGIATVREVLSD RELHLSWEVGKPRPPLNRNY VFTGYRVTKNSKVQIGEYTF EKGDYGDAVVYRGTTTYKLN VGDYFVLTSHTVMPLSAPT

Description

Background The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance