Anti-RFP (MOUSE) Monoclonal Antibody
Cat# 200-301-379
Size : 100ug
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Background
Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, Western blotting, immunohistochemistry, and flow cytometry. RFP Proteins are useful markers for imagining protein localization, monitoring physiological processes, and detecting transgenic expression. Rockland's anti-RFP antibody can be used to detect native RFP and RFP variants.
Specifications for RFP Antibody
Product Details
Anti-RFP (MOUSE) Monoclonal Antibody - 200-301-379
mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Mouse
Monoclonal
IgG2a
Target Details
DsRed - View All DsRed Products
RFP, mCherry, rRFP
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
UniProtKB - Q9U6Y8
Application Details
ELISA, WB
FISH, IF, IHC, Multiplex - View References
Monoclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA and Western blot, and is suitable for use in FISH, IF, IHC, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher.
Formulation
Liquid (sterile filtered)
1.02 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store anti-RFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
References (34)
Henriques VJ et al. (2022). Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex. Cells.
Applications
IHC, ICC, Histology PubMed
Zhang, L et al. (2022). Nutrients and pheromones promote insulin release to inhibit courtship drive. Science Advances
Applications
IF, Confocal Microscopy PubMed
Slupe AM et al. (2021). GABAergic neurons are susceptible to BAX-dependent apoptosis following isoflurane exposure in the neonatal period. PLoS One.
Applications
WB, IB, PCA PubMed
Hafner G et al. (2021). Increased Callosal Connectivity in Reeler Mice Revealed by Brain-Wide Input Mapping of VIP Neurons in Barrel Cortex. Cereb Cortex.
Applications
ISH, FISH PubMed
Vetere G et al. (2021). An inhibitory hippocampal-thalamic pathway modulates remote memory retrieval. Nat Neurosci.
Applications
IHC, ICC, Histology PubMed
Wu CH et al. (2021). Homeostatic synaptic scaling establishes the specificity of an associative memory. Curr Biol.
Applications
IHC, ICC, Histology PubMed
Hutton C et al. (2021). Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell.
Applications
Undefined PubMed
Xiao L et al. (2021). Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird. Nat Commun.
Applications
IHC, ICC, Histology; WB, IB, PCA PubMed
Hennes M, Lombaert N, Wahis J, Van den Haute C, Holt MG, Arckens L. (2020). Astrocytes shape the plastic response of adult cortical neurons to vision loss. Glia
Applications
IHC, ICC, Histology PubMed
Guan Z, Quiñones-Frías MC, Akbergenova Y, Littleton JT. (2020). Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent manner. Elife
Applications
WB, IB, PCA PubMed
Abecassis ZA, Berceau BL, Win PH, et al. (2020). Npas1+-Nkx2.1+ Neurons Are an Integral Part of the Cortico-pallido-cortical Loop. J Neurosci.
Applications
IF, Confocal Microscopy PubMed
Adrados CS et al. (2020). Salamander‐Eci: An optical clearing protocol for the three‐dimensional exploration of regeneration. Dev Dyn.
Applications
IF, Confocal Microscopy PubMed
Katrancha et al. (2019). Trio Haploinsufficiency Causes Neurodevelopmental Disease-Associated Deficits. Cell Reports
Applications
IF, Confocal Microscopy PubMed
Donovan LJ, Spencer WC, Kitt MM, et al. (2019). Lmx1b is required at multiple stages to build expansive serotonergic axon architectures. Elife.
Applications
IHC, ICC, Histology PubMed
Hafner G, Witte M, Guy J, et al. (2019). Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep.
Applications
IHC, ICC, Histology; IF, Confocal Microscopy PubMed
Bragado Alonso S, Reinert JK, Marichal N, et al. (2019). An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants. EMBO J.
Applications
IF, Confocal Microscopy; Multiplex Assay PubMed
Singh, S et al. (2019). A new Elf5Cre ERT 2- GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues. Febs Letters
Applications
IF, Confocal Microscopy PubMed
Winkowski et al. (2018). Orbitofrontal Cortex Neurons Respond to Sound and Activate Primary Auditory Cortex Neurons. Cerebral Cortex
Applications
IHC, ICC, Histology PubMed
Rider et al. (2018). The interactome of EBV LMP1 evaluated by proximity-based BioID approach. Virology
Applications
WB, IB, PCA PubMed
Vascak M, Jin X, Jacobs KM, Povlishock JT. (2018). Mild Traumatic Brain Injury Induces Structural and Functional Disconnection of Local Neocortical Inhibitory Networks via Parvalbumin Interneuron Diffuse Axonal Injury. Cereb Cortex.
Applications
IHC, ICC, Histology PubMed
Girasole AE, Lum MY, Nathaniel D, et al. (2018). A Subpopulation of Striatal Neurons Mediates Levodopa-Induced Dyskinesia. Neuron.
Applications
IF, Confocal Microscopy PubMed
Rieche F, Carmine-Simmen K, Poeck B, Kretzschmar D, Strauss R. (2018). Drosophila Full-Length Amyloid Precursor Protein Is Required for Visual Working Memory and Prevents Age-Related Memory Impairment. Curr Biol.
Applications
IF, Confocal Microscopy PubMed
Sin, YY et al. (2018). Transplantation of Gene-Edited Hepatocyte-like Cells Modestly Improves Survival of Arginase-1-Deficient Mice. Molecular Therapy. Nucleic Acids
Applications
WB, IB, PCA PubMed
Bifari et al. (2017). Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex. Cell Stem Cell
Applications
IHC, ICC, Histology PubMed
Clarkson et al. (2017). D3 Receptors Regulate Excitability in a Unique Class of Prefrontal Pyramidal Cells. The Journal of Neuroscience
Applications
IHC, ICC, Histology PubMed
Zhou et al. (2017). Subcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneurons. Cerebral Cortex
Applications
IHC, ICC, Histology PubMed
Meese et al. (2017). Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin. Frontiers in Synaptic Neuroscience
Applications
WB, IB, PCA PubMed
Hou et al. (2017). NMDA Receptors Regulate the Development of Neuronal Intrinsic Excitability through Cell-Autonomous Mechanisms. Frontiers in Cellular Neuroscience
Applications
IF, Confocal Microscopy PubMed
Guan et al. (2017). Eye opening differentially modulates inhibitory synaptic transmission in the developing visual cortex. Elife
Applications
IHC, ICC, Histology PubMed
Dixon RE, Vivas O, Hannigan KI, Dickson EJ. (2017). Ground State Depletion Super-resolution Imaging in Mammalian Cells. J Vis Exp.
Applications
IF, Confocal Microscopy PubMed
Hutton SR, Otis JM, Kim EM, Lamsal Y, Stuber GD, Snider WD. (2017). ERK/MAPK Signaling Is Required for Pathway-Specific Striatal Motor Functions. J Neurosci.
Applications
IHC, ICC, Histology PubMed
Wu et al. (2016). Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase. Journal of Biological Chemistry
Applications
WB, IB, PCA PubMed
Lebrigand, K et al. (2016). Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes. PloS Genetics
Applications
WB, IB, PCA PubMed
Clancy, JW et al. (2015). Regulated delivery of molecular cargo to invasive tumour-derived microvesicles. Nature Communications
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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