Anti-RAT IgG (H&L) (GOAT) Antibody ATTO 647N Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins)

Cat# 612-156-120S

Size : 100ug

Brand : Rockland Immunochemicals

Contact local distributor :


Phone : +1 850 650 7790

Background

Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Expand Background

Specifications for Rat IgG (H&L) Antibody ATTO 647N Conjugated Pre-Adsorbed

Product Details

Goat Anti-Rat IgG (H&L) Antibody ATTO 647N Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) - 612-156-120
Goat anti-Rat IgG ATTO647N Conjugated Antibody, Goat anti-Rat IgG Antibody ATTO 647N Conjugation
Goat
IgG (H&L)
ATTO 647N
Polyclonal
IgG

Target Details

Rat
Rat IgG whole molecule
Rat IgG (H&L) Antibody ATTO 647N was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.  This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.

Application Details

Dot Blot, WB
IF, IHC, Other, Multiplex  - View References
Anti-Rat IgG (H&L) conjugated to ATTO 647N has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Formulation

Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
500 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

References (7)

(). A neural network for wind-guided compass navigation. Neuron.
Applications
IF, Confocal Microscopy; Other
PubMed
(). Distributed control of motor circuits for backward walking in Drosophila. Nat Commun.
Applications
IHC, ICC, Histology
PubMed
(). Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body. Elife.
Applications
IF, Confocal Microscopy; IHC, ICC, Histology; Other
PubMed
(). Neuroarchitecture of the Drosophila central complex: A catalog of nodulus and asymmetrical body neurons and a revision of the protocerebral bridge catalog. The Journal of Comparative Neurology
Applications
WB, IB, PCA
PubMed
(). Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons. PLOS One
Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
(). Angular velocity integration in a fly heading circuit. Elife
Applications
IHC, ICC, Histology
PubMed
(). Feature Integration Drives Probabilistic Behavior in the Drosophila Escape Response. Neuron
Applications
IF, Confocal Microscopy; Multiplex Assay
PubMed

Related Protocols

Adherent Cell Lysis Protocol
Fluorescent Western Blotting Protocol
General Immunoblotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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