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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 800 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)

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Cat# 611-145-122

Size : 100ug

Contact local distributor :

Phone : +1 850 650 7790

Brand : Rockland Immunochemicals

Contact local distributor :

Phone : +1 850 650 7790

Background

Conjugated DyLight™ 800 secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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Specifications for Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated Pre-Adsorbed

Product Details

Description: Goat Anti-Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-145-122

Synonyms: Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody

Host Species: Goat

Specificity: IgG (H&L)

Conjugate: DyLight™ 800

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Rabbit

Immunogen: Rabbit IgG whole molecule

Purity/Specificity: This DyLight™ 800 conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.

Application Details

Tested Applications: Dot Blot

Suggested Applications: Other, WB - View References

Application Note: Anti-Rabbit IgG DyLight™800 has been tested by dot blot. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (18)

Joshi, H et al. (2022). L-plastin enhances NLRP3 inflammasome assembly and bleomycin-induced lung fibrosis. Cell Reports

Applications

WB, IB, PCA

PubMed

Xue J et al. (2021). Caffeine improves bladder function in diabetic rats via a neuroprotective effect. Exp Ther Med.

Applications

WB, IB, PCA

PubMed

Zhang P et al. (2021). Atorvastatin alleviates microglia-mediated neuroinflammation via modulating the microbial composition and the intestinal barrier function in ischemic stroke mice. Free Radic Biol Med.

Applications

WB, IB, PCA

PubMed

Chen J et al. (2021). Inhibition of Acyl-CoA Synthetase Long-Chain Family Member 4 Facilitates Neurological Recovery After Stroke by Regulation Ferroptosis. Front Cell Neurosci.

Applications

WB, IB, PCA

PubMed

Tichy ED et al. (2021). Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening. Cell Rep.

Applications

WB, IB, PCA

PubMed

Navarro R et al. (2020). TGF‐β‐induced IGFBP‐3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion. Mol Oncol.

Applications

WB, IB, PCA

PubMed

Mitra S, Bodor DL, David AF, et al. (2020). Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin. Nat Commun.

Applications

WB, IB, PCA

PubMed

Takahashi H, Ranjan A, Chen S, et al. (2020). The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun.

Applications

WB, IB, PCA

PubMed

Acin-Perez R et al. (2020). Analyzing electron transport chain supercomplexes. Methods Cell Biol.

Applications

WB, IB, PCA

PubMed

Mastro TL et al. (2020). A sex difference in the response of the rodent postsynaptic density to synGAP haploinsufficiency. Elife.

Applications

WB, IB, PCA

PubMed

McManus MJ et al. (2019). Mitochondrial DNA variation dictates expressivity and progression of nuclear DNA mutations causing cardiomyopathy. Cell Metab.

Applications

WB, IB, PCA

PubMed

Latorre-Pellicer A et al. (2019). Regulation of mother-to-offspring transmission of mtDNA heteroplasmy. Cell Metab.

Applications

WB, IB, PCA

PubMed

Chen J et al. (2019). Ginsenoside Rg1 promotes cerebral angiogenesis via the PI3K/Akt/mTOR signaling pathway in ischemic mice. Eur J Pharmacol.

Applications

WB, IB, PCA

PubMed

Pereira et al. (2018). mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels. EMBO Molecular Medicine

Applications

WB, IB, PCA

PubMed

Nguyen et al. (2017). FLT3 activating mutations display differential sensitivity to multiple tyrosine kinase inhibitors. Oncotarget

Applications

WB, IB, PCA

PubMed

Meyer et al. (2016). Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer. Biochemistry and Biophysics Reports

Applications

WB, IB, PCA

PubMed

Wu JW et al. (2016). Neuronal activity enhances tau propagation and tau pathology in vivo. Nat Neurosci.

Applications

WB, IB, PCA

PubMed

Klammer M et al. (2012). Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics.

Applications

WB, IB, PCA

PubMed

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Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.