Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000 IHC 1:100-1:300 ELISA 1:40000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	NR4A1
Gene ID:	3164
Uniprot ID:	NR4A1_HUMAN
Immunogen Region:	317-366 aa
Specificity:	Phospho-Nur77 (S351) Polyclonal Antibody detects endogenous levels of Nur77 protein only when phosphorylated at S351.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Nuclear Receptor NR4A1 around the phosphorylation site of Ser351 at the amino acid range 317-366

Post Translational Modifications	Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli. Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1.
Function	Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3'. Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter. May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration. Plays a role in the vascular response to injury. In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7.
Protein Name	Nuclear Receptor Subfamily 4immunitygroup A Member 1 Early Response Protein Nak1 Nuclear Hormone Receptor Nur/77 Nur77 Orphan Nuclear Receptor Hmr Orphan Nuclear Receptor Tr3 St-59 Testicular Receptor 3
Database Links	Reactome: R-HSA-198693 Reactome: R-HSA-383280 Reactome: R-HSA-5674400
Cellular Localisation	Nucleus Cytoplasm Cytosol Mitochondrion Nuclear Export To The Cytosol Is Xpo1-Mediated And Positively Regulated By Ifi27 Translocation To The Mitochondrion Upon Interaction With Rxra And Upon The Presence Of 9-Cis Retinoic Acid
Alternative Antibody Names	Anti-Nuclear Receptor Subfamily 4immunitygroup A Member 1 antibody Anti-Early Response Protein Nak1 antibody Anti-Nuclear Hormone Receptor Nur/77 antibody Anti-Nur77 antibody Anti-Orphan Nuclear Receptor Hmr antibody Anti-Orphan Nuclear Receptor Tr3 antibody Anti-St-59 antibody Anti-Testicular Receptor 3 antibody Anti-NR4A1 antibody Anti-GFRP1 antibody Anti-HMR antibody Anti-NAK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance