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> Anti-p35/Cdk5 (RABBIT) Antibody

Anti-p35/Cdk5 (RABBIT) Antibody

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Cat# 600-401-ME9

Size : 100ug

Brand : Rockland Immunochemicals

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Phone : +1 850 650 7790

Background

p35, is a neuron specific activator of CDK5. Also known as Cdk5r1 (Cyclin-dependent kinase 5 activator 1), the complex p35/CDK5 is required for neurite outgrowth and cortical lamination. It is involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at Thr-451 and Thr-461 and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution. Diseases associated with CDK5R1 include Alzheimer Disease and Non-Syndromic Intellectual Disability. Anti-p35/Cdk5 Antibody is useful for researchers interested in Alzheimer researcher, protein kinase binding, Neuroscience Research, and Cancer Research.

Expand Background

Specifications for p35 Antibody

Product Details

Anti-p35/Cdk5 (RABBIT) Antibody - 600-401-ME9
Rabbit Anti-Cyclin Dependent Kinase 5 Regulatory Subunit 1 Antibody, Rabbit Anti-Cyclin-dependent kinase 5 activator 1 Antibody, Rabbit Anti-p35 Antibody, TPKII Regulatory Subunit, CDK5 Activator 1, CDK5R, NCK5A, Cyclin-Dependent Kinase 5, Regulatory Subunit 1 (P35), Cyclin-Dependent Kinase 5 Regulatory Subunit 1, Cyclin-Dependent Kinase 5 Activator 1, Tau Protein Kinase II 23kDa Subunit, Regulatory Partner For CDK5 Kinase, Neuronal CDK5 Activator, P35nck5a, CDK5P35, P23, P25, P35 
Rabbit
Polyclonal
IgG

Target Details

Cdk5r1  - View All Cdk5r1 Products
Human, Mouse
Conjugated Peptide
Anti-p35/Cdk5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse p35/Cdk5 conjugated to Keyhole Limpet Hemocyanin (KLH).
This affinity purified antibody is directed against mouse p35/Cdk5.  This product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to rat, human, bovine, and Spermophilus citellus.
UniProtKB - P61809
NCBI - NP_034001.1
GeneID - 12569

Application Details

ELISA, IHC, WB
Anti-p35/Cdk5 Antibody has been tested in ELISA, WB, and IHC.  Expect bands ~34 kDa in western blot using appropriate tissues and lysates.  Positive control used: mouse brain lysate in WB and IHC. This antibody is suitable in IF.

Formulation

Liquid (sterile filtered)
0.96 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

Certificate of Analysis Lookup

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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