Anti-Otoferlin Antibody Picoband™

Cat# PB9616

Size : 100ug/vial

Brand : Boster Immunoleader

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Phone : +1 850 650 7790

  • pb9616 1 WB anti otoferlin picoband antibody
  • pb9616 2 IHC anti otoferlin picoband antibody
  • pb9616 3 IHC anti otoferlin picoband antibody
  • pb9616 4 IHC anti otoferlin picoband antibody

Product Info Summary

SKU: PB9616
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product info

Product Name

Anti-Otoferlin Antibody Picoband™

View all Otoferlin Antibodies

SKU/Catalog Number

PB9616

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Otoferlin Antibody Picoband™ catalog # PB9616. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Otoferlin Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9616)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Otoferlin, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9616 is reactive to OTOF in Human, Mouse, Rat

Applications

PB9616 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

227 kDa

Calculated molecular weight

226.753kDa

Background of Otoferlin

Otoferlin is a protein that in humans is encoded by the OTOF gene. Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has three C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has six C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multipleisoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay dilution & Images

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Protein Target Info & Infographic

Gene/Protein Information For OTOF (Source: Uniprot.org, NCBI)

Gene Name

OTOF

Full Name

Otoferlin

Weight

226.753kDa

Superfamily

ferlin family

Alternative Names

AUNB1; DFNB6; DFNB9; EC 2.7.10.1; EC 4.2.1.11; FER1L2NSRD9; Fer-1-like protein 2; otoferlin OTOF AUNB1, DFNB6, DFNB9, FER1L2, NSRD9 otoferlin otoferlin|fer-1-like family member 2|fer-1-like protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".