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> Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) Antibody

Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) Antibody

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Cat# p1311-53t

Size : 25uL

Brand : Phosphosolutions

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Phone : +1 850 650 7790

Anti-NCC (Thiazide Sensitive NaCl Cotransporter) (Thr53) Antibody

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Our Anti-NCC (Thiazide sensitive NaCl cotransporter) (Thr53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NCC (Thiazide sensitive NaCl cotransporter) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat. p1311-53, red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
Two Western blots show the abundance of pNCCT53 (cat. p1311-53, 1:200) and tNCC in male Kcnj10fl/fl mice and in Ks-Kir4.1–KO mice treated with vehicle (control) and FK506 (0.75 mg/kg BW). Image from publication CC-BY-4.0. PMID: 36821372
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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