Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins)
Cat# 610-1319
Size : 2mg
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Background
Anti-Mouse IgG Peroxidase Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Specifications for Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed
Product Details
Goat Anti-Mouse IgG (H&L) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) - 610-1319
Goat Anti-Mouse IgG Secondary Antibody Peroxidase Conjugated, Goat Anti-Mouse IgG Secondary Antibody HRP Conjugated, GAM-HRP, Anti-mouse secondary antibody, anti-mouse HRP antibody, horseradish peroxidase conjugated secondary antibody, anti-mouse horseradish peroxidase conjugated secondary antibody
Goat
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG
Target Details
Mouse
Mouse IgG whole molecule
HRP Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
Application Details
ELISA
IHC, Other, WB - View References
Mouse secondary antibody conjugated to peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for Immunohistochemistry, Western blotting as well as other antibody based assays.
Formulation
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
References (47)
Srivastav S et al. (2024). Motor neuron activity enhances the proteomic stress caused by autophagy defects in the target muscle. PLoS One.
Applications
WB, IB, PCA PubMed
van der Graaf K et al. (2024). The Drosophila Nesprin-1 homolog MSP300 is required for muscle autophagy and proteostasis. J Cell Sci.
Applications
WB, IB, PCA PubMed
Castro JJ et al. (2023). DHA Supplementation during Pregnancy in Women with Obesity Normalizes IGF2R Levels in the Placenta of Male Newborns. Int J Endocrinol.
Applications
WB, IB, PCA PubMed
Ramirez E et al. (2023). Discovery of 4-aminoindole carboxamide derivatives to curtail alpha-synuclein and tau isoform 2N4R oligomer formation. Results Chem.
Applications
WB, IB, PCA PubMed
Chen Y et al. (2023). Unannotated microprotein EMBOW regulates the interactome and chromatin and mitotic functions of WDR5. Cell Rep.
Applications
WB, IB, PCA PubMed
Cao X et al. (2022). Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint inhibitor. Nat Chem Biol.
Applications
WB, IB, PCA PubMed
Na Z et al. (2022). Mapping subcellular localizations of unannotated microproteins and alternative proteins with MicroID. Mol Cell.
Applications
Undefined PubMed
Shrestha RL et al. (2021). CENP-A overexpression promotes aneuploidy with karyotypic heterogeneity. J Cell Biol.
Applications
WB, IB, PCA PubMed
Guo Y et al. (2021). p53 isoforms differentially impact on the POLι dependent DNA damage tolerance pathway. Cell Death Dis.
Applications
WB, IB, PCA PubMed
Na Z et al. (2021). Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates. J Am Chem Soc.
Applications
WB, IB, PCA PubMed
Glanz A et al. (2021). Autophagic degradation of IRF3 induced by the small-molecule auranofin inhibits its transcriptional and proapoptotic activities. J Biol Chem.
Applications
WB, IB, PCA PubMed
Chowdhury D et al. (2021). Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity. Front Immunol.
Applications
WB, IB, PCA PubMed
Luo Y et al. (2020). Global profiling of cellular substrates of human Dcp2. Biochemistry.
Applications
WB, IB, PCA PubMed
Merlet AN et al. (2020). Muscle structural, energetic and functional benefits of endurance exercise training in sickle cell disease. Am J Hematol.
Applications
WB, IB, PCA PubMed
Gruber CN et al. (2020). Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell.
Applications
Microneutralization Assay (MNT) PubMed
Na Z et al. (2020). The NBDY microprotein regulates cellular RNA decapping. Biochemistry.
Applications
WB, IB, PCA PubMed
Luo Y et al. (2020). Discovery of cellular substrates of human RNA-decapping enzyme DCP2 using a stapled bicyclic peptide inhibitor. Cell Chem Biol.
Applications
WB, IB, PCA PubMed
Monette A. et al. (2020). Pan-retroviral Nucleocapsid-Mediated Phase Separation Regulates Genomic RNA Positioning and Trafficking. Cell Rep.
Applications
WB, IB, PCA PubMed
Roscoe S et al. (2020). Formation of mRNP granules in Toxoplasma gondii during the lytic cycle. Mol Biochem Parasitol.
Applications
WB, IB, PCA PubMed
Granger MW et al. (2019). Distinct disruptions in Land's cycle remodeling of glycerophosphocholines in murine cortex mark symptomatic onset and progression in two Alzheimer's disease mouse models. J Neurochem.
Applications
WB, IB, PCA PubMed
O'Neill et al. (2018). The 3' UTRs of Brain-Derived Neurotrophic Factor Transcripts Differentially Regulate the Dendritic Arbor. Frontiers in Cellular Neuroscience
Applications
WB, IB, PCA PubMed
Crater AK et al. (2018). Toxoplasma ubiquitin-like protease 1, a key enzyme in sumoylation and desumoylation pathways, is under the control of non-coding RNAs. Int J Parasitol.
Applications
WB, IB, PCA PubMed
D'Lima et al. (2017). A human microprotein that interacts with the mRNA decapping complex. Nature Chemical Biology
Applications
WB, IB, PCA PubMed
Castaño et al. (2017). Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLOS One
Applications
WB, IB, PCA PubMed
Fernandez-Verdejo R et al. (2017). Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training. FASEB J.
Applications
WB, IB, PCA PubMed
Shrestha RL et al. (2017). Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget.
Applications
WB, IB, PCA PubMed
Ciesielska A et al. (2017). Bis (monoacylglycero) phosphate inhibits TLR4-dependent RANTES production in macrophages. Int J Biochem Cell Biol.
Applications
EIA, ELISA PubMed
Granger MW et al. (2016). A TgCRND8 mouse model of Alzheimer's disease exhibits sexual dimorphisms in behavioral indices of cognitive reserve. J Alzheimers Dis.
Applications
WB, IB, PCA PubMed
Crater AK et al. (2015). Utilization of inherent miRNAs in functional analyses of Toxoplasma gondii genes. J Microbiol Methods.
Applications
WB, IB, PCA PubMed
Hashimoto H et al. (2014). Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA. Nature.
Applications
WB, IB, PCA PubMed
Cherry AA et al. (2014). Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules. Gene.
Applications
WB, IB, PCA PubMed
Ray, B et al. (2014). Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research. Molecular Brain
Applications
WB, IB, PCA PubMed
Wang F et al. (2013). Phosphoproteome analysis of an early onset mouse model (T g CRND 8) of A lzheimer's disease reveals temporal changes in neuronal and glia signaling pathways. Proteomics.
Applications
WB, IB, PCA PubMed
Frasca D et al. (2012). A molecular mechanism for TNF-α–mediated downregulation of B cell responses. J Immunol.
Applications
WB, IB, PCA PubMed
Horn P et al. (2012). Circulating microparticles carry a functional endothelial nitric oxide synthase that is decreased in patients with endothelial dysfunction. J Am Heart Assoc.
Applications
WB, IB, PCA PubMed
LeVine H et al. (2012). Dihydroxybenzoic acid isomers differentially dissociate soluble biotinyl-Aβ(1-42) oligomers. Biochemistry
Applications
E, EIA PubMed
Zhang X et al. (2011). Vaccination with different M2e epitope densities confers partial protection against H5N1 influenza A virus challenge in chickens. Intervirology.
Applications
WB, IB, PCA PubMed
McCall LI et al. (2010). Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. Mol Microbiol.
Applications
WB, IB, PCA PubMed
Frasca D et al. (2010). Protein phosphatase 2A (PP2A) is increased in old murine B cells and mediates p38 MAPK/tristetraprolin dephosphorylation and E47 mRNA instability. Mech Ageing Dev.
Applications
WB, IB, PCA PubMed
Reeves TM et al. (2010). Proteolysis of submembrane cytoskeletal proteins ankyrin-G and αII-spectrin following diffuse brain injury: a role in white matter vulnerability at Nodes of Ranvier. Brain Pathology (Zurich, Switzerland)
Applications
WB, IB, PCA PubMed
Ronsyn MW et al. (2007). Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord. BMC Biotechnol.
Applications
IHC, ICC, Histology PubMed
Frasca D et al. (2005). RNA stability of the E2A-encoded transcription factor E47 is lower in splenic activated B cells from aged mice. J Immunol.
Applications
WB, IB, PCA PubMed
Van Komen JS et al. (2005). The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo. Eukaryot Cell.
Applications
WB, IB, PCA PubMed
Frasca D et al. (2004). Reduced Ig class switch in aged mice correlates with decreased E47 and activation-induced cytidine deaminase. J Immunol.
Applications
WB, IB, PCA PubMed
Frasca D et al. (2004). Age-related differences in the E2A-encoded transcription factor E47 in bone marrow-derived B cell precursors and in splenic B cells. Exp Gerontol.
Applications
WB, IB, PCA PubMed
Frasca D et al. (2003). Effects of aging on proliferation and E47 transcription factor activity induced by different stimuli in murine splenic B cells. Mech Ageing Dev.
Applications
WB, IB, PCA PubMed
Frasca D et al. (2003). The age-related decrease in E47 DNA-binding does not depend on increased Id inhibitory proteins in bone marrow-derived B cell precursors. Front Biosci.
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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