Anti-Mouse/Human KLRG-1 - FITC

IL2activated NK cells (ALAK cells) from B6 (H2b) mice

This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Liquid

This Fluorescein conjugate is stable when stored at 28°C. Do not freeze.
For longer term storage, aseptically aliquot in working volumes without diluting and store at 80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

Blue Laser (488 nm)

2F1 activity is directed against mouse and human KLRG1.

Killer cell lectinlike receptor G1 (KLRG1) is a transmembrane glycoprotein with an extracellular Ctype lectinlike domain and a cytoplasmic immunoreceptor tyrosinebased inhibitory motif (ITIM)¹. KLRG1 plays an inhibitory role in human NK and T cells² and is frequently used as a marker of cellular differentiation, with memory cells and highly differentiated endstage cells having a significantly higher percentage of expression than differentiating cells². Additionally, the proportion of CD8⁺ T cells expressing KLRG1 is agerelated, increasing from ~ 40% of the CD8⁺ T cell pool in young adults to 90% in individuals over 65. KLRG1 is known to bind E, N, and Rcadherin^{1, 2}. Cadherin interactions are generally involved in celltocell adhesion and additionally, KLRG1/Ecadherin interactions are involved in immune control².

2F1 was generated by immunizing Syrian hamsters with IL2 activated NK (ALAK) cells from B6 (H2^b) mice³. Popliteal lymph node cells were fused to murine myeloma P3X63Ag.8.653 cells and hybridomas screened for antibodies specific to ALAK cells.

Antibody 2F1 was used to show that KLRG1 is indirectly upregulated by expression of MHC class I molecules in an interaction affected by class Ispecific Ly49 inhibitory receptors and was also used to show that KLRG1 expression is modulated by SHP1 ³ . Additionally, 2F1 was used to identify the KLRG1 mouse homolog and show that it is not expressed on mouse mast cells ⁴.

KLRG1 is expressed by subsets of natural killer cells and a small fraction of T cells but not by other lymphohematopoietic cells in both mouse and human.

E, N, R cadherins

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4. Hanke T, Corral L, Vance RE, et al. Eur J Immunol. 28(12):44094417. 1998.
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8. Menendez CM, Jinkins JK, Carr DJ. J Immunol. 197(4):12621275. 2016.
9. Graham JB, Swarts JL, Lund JM. Curr Protoc Mouse Biol. 7(4):221235. 2017.
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11. Yasuda K, Kitagawa Y, Kawakami R, et al. Nat Commun. 10(1):549. 2019.
12. Garg G, Muschaweckh A, Moreno H, et al. Cell Rep. 26(7):18541868.e5. 2019.
13. Li A, Herbst RH, Canner D, et al. Cell Rep. 29(10):29983008.e8. 2019.
14. Sidwell T, Liao Y, Garnham AL, et al. Nat Commun. 11(1):252. 2020.
15. Vasanthakumar A, Chisanga D, Blume J, et al. Nature. 579(7800):581585. 2020.
16. Huang RY, Francois A, McGray AR, et al. Oncoimmunology. 6(1):e1249561. 2016.
17. Zhao M, Shao F, Yu D, et al. Nat Commun. 13(1):7600. 2022.