Anti-LRRK2/Dardarin (MOUSE) Monoclonal Antibody Fluorescein Conjugated

Cat# 200-302-W59

Size : 100ug

Brand : Rockland Immunochemicals

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Phone : +1 850 650 7790

Background

LRRK2 is a large protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named Roc, and a kinase domain that is closely related to the RIP kinase domain. LRRK2 gene is expressed in brain as well as in other tissues such as lung, liver and heart. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with parkinsonism. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features. Anti-LRRK2 is ideal for researchers interested in Neuroscience, specifically parkinsonism, as well as pathways related to the Electron Transport Chain and Wnt Signaling.

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Specifications for LRRK2/Dardarin Antibody Fluorescein

Product Details

Anti-LRRK2/Dardarin (MOUSE) Monoclonal Antibody Fluorescein Conjugated - 200-302-W59
Leucine-rich repeat kinase 2, RIP7, PARK8, Dardarin, ROCO 2, RIPK7, Leucine-rich repeat serine/threonine-protein kinase 2, augmented in rheumatoid arthritis 29
Mouse
Fluorescein (FITC)
Monoclonal
IgG2a

Target Details

LRRK2  - View All LRRK2 Products
Human, Rat
Recombinant Protein
Anti-LRRK2/Dardarin Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 841-960 of human LRRK2.
Anti-LRRK2/Dardarin Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. BLAST analysis suggests 81% similarity to mouse, 80% similarity to rat, and <30% identical to LRRK1.
UniProtKB - Q5S007
GeneID - 120892

Application Details

IHC, WB
Anti-LRRK2/Dardarin FITC Conjugated Antibody is tested for Western Blots, Immunocytochemistry, and Immunohistochemistry. Expect a band approximately >200 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Liquid (sterile filtered)
1mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
50% (v/v) Glycerol

Shipping & Handling

Wet Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Related Protocols

Adherent Cell Lysis Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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