Anti-Human PD-ECGF
Cat# P264-100
Size : 100µg
Brand : Leinco Technologies
Contact local distributor :
Phone : +1 850 650 7790
AntiHuman PDECGF
AntiHuman PDECGF
Product No.: P264
Target PDECGF Product Type Polyclonal Antibody Alternate Names Endothelial Cell Growth Factor1 (ECGF1), Gliostatin, PlateletDerived Endothelial Cell Migration Applications IHC FFPE , N , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Sf21derived human PDECGF Endotoxin Level <0.01 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at 20°C to 70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831560 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human PDECGF this polyclonal antibody can be used at a concentration of 0.10.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible secondstep reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human PDECGF is 5 ng/lane under either reducing or nonreducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P252. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 515 µg/ml. The recommended secondary antibody for IHC is PN:G505. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human PDECGF bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human PDECGF (at 150 ng/ml) is 0.52.0 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat AntiHuman PlateletDerived Endothelial Cell Growth Factor (PDECGF) recognizes Human PDECGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background PlateletDerived Endothelial Cell Growth Factor (PDECGF) is an acidic nonglycosylated protein found in connective tissue of the placenta. It is synthesized by fibroblasts, vascular smooth muscle cells, and a number of transformed cell lines.1 PDGF promotes the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs), and may play a role in the progression of pulmonary arterial hypertension (PAH), a condition characterized by proliferation of PASMCs resulting in the obstruction of small pulmonary arteries.2 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Miyazono, K. et al. (1991) Progress in Growth Factor Res. 3: 207 2. Humbert, M. et al. (2008) Am J Respir Crit Care Med. 178: 81 |