Anti-Human CD57 (Clone VC1.1) - APC

This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This APC conjugate is stable when stored at 28°C. Do not freeze.

Next Day 28°C

Red Laser (650 nm)

Mouse AntiHuman CD57 (Clone VC1.1) recognizes Human CD57. This monoclonal antibody was purified using multistep affinity chromatography methods such as Protein A or G depending on the species and isotype. AntiCD57 recognizes a (Mr 110 kD) carbohydrate molecule on myeloid cells. This surface antigen is associated with myelinassociated glycoprotein (MAG).¹ This antibody blocks binding of HNK1 (Leu. 7) antibody.³

AntiCD57 may be used in flow cytometry, Western blotting and immunohistology on formalin fixed paraffin embedded tissue sections. The antibody is useful for enumeration of NK subsets and Tlymphocyte subsets, studies of neural tissue, malignant tissues and AIDSrelated complex.

The CD57 antigen is present on 1520% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of Tlymphocytes. This carbohydrate is also present on NCAM in the nervous system.

Binds to Lselectin and Pselectin in a calciumdependent manner, also binds to second globular domain of E8 laminin fragment.

1. Barnstable, C. et al. (1995) Brain Research 559:118 2. Leukocyte Typing V (1995) Oxford University Press: Code No. NK67 3. Arimatsu, Y. et al. (1987) J. Neuroscience 7:1250 4. Abo, T., and Balch, C.M. et al. (1981) J. Immunol. 127:1024