Immunohistochemical staining of Giant Cell Bone Tumor (GCBT) Image courtesy of Hidetaka Yamamoto, MD, PhD, Immunohistochemical staining of formalin fixed and Western Blot analysis of cell lysates prepared from
Product Details Product Name Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307 Product Description Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant Catalog No. 31-1193-00-S;
31-1193-00-L;
31-1193-02 Clone Name RM307 Product Data Sheet Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
Biotinylated Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307 Specificity RM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 . Immunogen A peptide corresponding to Histone H3.3 G34V mutant Application Western Blot
ELISA
Immunohistochemistry
Species Reactivity All Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Size 100 µL;
400 µL;
Biotinylated 50 µL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage Western Blot: 1:1000 – 1:10000;
ELISA: 1:500 – 1:5000;
IHC: 1:100 - 1:500.
References for Histone H3.3 G34V Mutant antibody [RM307] Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184)
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604)
Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742) . Product Images
tissue sections using Anti-Histone H3.3 G34V antibody, Clone RM307.
Kyushu University, Fukuoka, Japan
paraffin embedded 293T cells transfected with a
DNA construct encoding Histone H3.3 wild type
or G34V mutant, stained with anti-Histone H3.3
G34V rabbit monoclonal antibody, Clone RM307.
293T, transfected with a DNA construct encoding
wild type or G34V mutant proteins of Histone H3.3,
using anti-Histone H3.3 G34V rabbit monoclonal
antibody, Clone RM307.
Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [RM307]
![Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [RM307]](https://www.biotrend.com/en/upload/-img-lapin1.png)
Cat# 31-1193-00-S
Size : 100ul
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Histone H3.3 G34V;
Histone H3.3 G34V Mutant
50 µL Biotin Conjugate, 100 µL Small Size, 400 µL Large Size
Request Quote for Bulk Sizes