Anti-GFP (MOUSE) Monoclonal Antibody
Cat# 600-301-215M
Size : 100ug
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Background
Mouse anti-GFP antibody is functional by western blot, ELISA, Immunofluorescence Microscopy and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Specifications for GFP Monoclonal Antibody
Product Details
Anti-GFP (MOUSE) Monoclonal Antibody - 600-301-215
mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Mouse
Monoclonal
IgG
Target Details
GFP, eGFP, rGFP
Recombinant Protein
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215) from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
UniProtKB - P42212
Application Details
Dot Blot, ELISA, IP, WB
ChIP, FC, IF, IHC, Multiplex - View References
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Formulation
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
References (30)
Singh, A et al. (2022). Tcf12 and NeuroD1 cooperatively drive neuronal migration during cortical development. Development (Cambridge, England)
Applications
IHC, ICC, Histology PubMed
Albecka A et al. (2021). A functional assay for serum detection of antibodies against SARS-CoV-2 nucleoprotein. EMBO J.
Applications
FC, FACS, FLOW; WB, IB, PCA PubMed
Kumar S et al. (2021). The neural G protein Gαo tagged with GFP at an internal loop is functional in Caenorhabditis elegans. G3 (Bethesda).
Applications
IP, Co-IP PubMed
Garg T. et al. (2020). Genome-Wide High Resolution Expression Map and Functions of Key Cell Fate Determinants Reveal the Dynamics of Crown Root Development in Rice. bioRxiv.
Applications
IHC, ICC, Histology Source
Sundaramurthy S. et al. (2020). FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner. Cytoskeleton (Hoboken).
Applications
IF, Confocal Microscopy PubMed
Santos M et al. (2020). Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype. J Clin Med.
Applications
IP, Co-IP PubMed
Zaja R et al. (2020). Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome. Sci Rep.
Applications
WB, IB, PCA PubMed
Courtney AJ et al. (2020). Normal Patterns of Histone H3K27 Methylation Require the Histone Variant H2A.Z in Neurospora crassa. Genetics.
Applications
ChIP PubMed
Khlghatyan J et al. (2020). Fxr1 regulates sleep and synaptic homeostasis. EMBO J.
Applications
WB, IB, PCA PubMed
Tian Y et al. (2020). The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1. 1 through protein kinase C delta. BMC Biol.
Applications
WB, IB, PCA PubMed
Yao Y et al. (2020). Phase Coupled Firing of Prefrontal Parvalbumin Interneuron With High Frequency Oscillations. Front Cell Neurosci.
Applications
IF, Confocal Microscopy PubMed
Dohmen M et al. (2020). AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy. Nat Commun.
Applications
WB, IB, PCA PubMed
Santos M, Morais S, Pereira C, Sequeiros J, Alonso I. (2019). Parkin truncating variants result in a loss-of-function phenotype. Sci Rep.
Applications
IP, Co-IP PubMed
Brito, C et al. (2019). Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum Redistribution. Toxins
Applications
TEM PubMed
Liu, W et al. (2019). Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope. Journal of Molecular Cell Biology
Applications
WB, IB, PCA; IP, Co-IP PubMed
Khlghatyan et al. (2018). Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3β Are Modulators of Anxiety and Glutamatergic Neurotransmission. Frontiers in Molecular Neuroscience
Applications
WB, IB, PCA PubMed
Nicholson DA, Roberts TF, Sober SJ. et al. (2018). Thalamostriatal and cerebellothalamic pathways in a songbird, the Bengalese finch. J Comp Neurol.
Applications
IF, Confocal Microscopy PubMed
Terrell et al. (2015). Nano-guided cell networks as conveyors of molecular communication. Nature Communications
Applications
IF, Confocal Microscopy PubMed
Reiner, A et sl. (2014). Motor, visual and emotional deficits in mice after closed-head mild traumatic brain injury are alleviated by the novel CB2 inverse agonist SMM-189. International Journal of Molecular Sciences
Applications
IF, Confocal Microscopy PubMed
Moerdyk-Schauwecker M et al. (2013). Resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus: role of type I interferon signaling. Virology.
Applications
WB, IB, PCA PubMed
Feijs KL et al. (2013). ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation. Cell Commun Signal.
Applications
WB, IB, PCA PubMed
Forst AH et al. (2013). Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains. Structure.
Applications
WB, IB, PCA PubMed
Hastie E et al. (2013). Oncolytic vesicular stomatitis virus in an immunocompetent model of MUC1-positive or MUC1-null pancreatic ductal adenocarcinoma. J Virol.
Applications
WB, IB, PCA PubMed
Vladimir A Volkov et al. (2013). Long tethers provide high-force coupling of the Dam1 ring to shortening microtubules. Proc Natl Acad Sci U S A.
Applications
Bead Conjugation PubMed
Sebastian, K et al. (2013). Characterization of SLCO5A1/OATP5A1, a solute carrier transport protein with non-classical function. PloS One
Applications
WB, IB, PCA PubMed
Goel M et al. (2012). Cochlin, Intraocular Pressure Regulation and Mechanosensing. PLoS One.
Applications
IHC, ICC, Histology; Multiplex Assay PubMed
Goel M et al. (2011). Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility. PLoS One.
Applications
IHC, ICC, Histology; Multiplex Assay PubMed
Jesse S et al. (2010). Lef‐1 isoforms regulate different target genes and reduce cellular adhesion. Int J Cancer.
Applications
ChIP PubMed
Zanger RC et al. (2009). ProMAT Calibrator: a tool for reducing experimental bias in antibody microarrays. J Proteome Res.
Applications
EIA, ELISA PubMed
Koenig A et al. (2006). Collagen Type I Induces disruption of E-cadherin–mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells. Cancer Res.
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
Bifunctional Cross-Linking Protocol
Chromatin Immunoprecipitation (ChIP) Protocol
ELISA Protocol
Flow Cytometry (FC) Protocol
Fluorescent Western Blotting Protocol
General Immunoblotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Nucleosome ChIP Protocol
Protease-induced Antigen Retrieval Protocol
Re-ChIP Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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