Anti-BrdU (MOUSE) Monoclonal Antibody

Cat# 200-301-H50

Size : 100ug

Brand : Rockland Immunochemicals

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Phone : +1 850 650 7790

Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.

Expand Background

Specifications for BrdU Antibody

Product Details

Anti-BrdU (MOUSE) Monoclonal Antibody - 200-301-H50
mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU
Mouse
Monoclonal
IgG

Target Details

BrdU, CldU, IdU
Other
Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.

Application Details

Dot Blot, IF
FISH, IHC  - View References
Anti-BrdU Antibody has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.

Formulation

Liquid (sterile filtered)
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

References (3)

(). Heterogeneity of Organization of Subcompartments in DSB Repair Foci. Frontiers in Genetics
Applications
IF, Confocal Microscopy
PubMed
(). Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis. Cell
Applications
IHC, ICC, Histology
PubMed
(). Parvovirus Expresses a Small Noncoding RNA That Plays an Essential Role in Virus Replication. Journal of Virology
Applications
IF, Confocal Microscopy; ISH, FISH
PubMed

Related Protocols

Adherent Cell Lysis Protocol
General Immunoblotting Protocol
Heat-induced Antigen Retrieval Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
Protease-induced Antigen Retrieval Protocol
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol

Certificate of Analysis Lookup

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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