Alpha-Melanocyte Stimulating Hormone ELISA Kit (Human)
Cat# OKCD02253
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Alpha-Melanocyte Stimulating Hormone ELISA Kit (Human) (OKCD02253) |
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| Predicted Species Reactivity | Human | ||||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||||
| ELISA Kit Duration | 2h | ||||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Melanocyte Stimulating Hormone (aMSH) and unlabeled Alpha-Melanocyte Stimulating Hormone (aMSH) (Standards or samples) with the pre-coated antibody specific to Alpha-Melanocyte Stimulating Hormone (aMSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Melanocyte Stimulating Hormone (aMSH) in the sample. | ||||||||||||||||||||||||
| ELISA Kit Range | 123.5-10,000pg/mL | ||||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Melanocyte Stimulating Hormone (aMSH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 581-05-5 PubChem: 16132636 | ||||||||||||||||||||||||
| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | ||||||||||||||||||||||||
| Sensitivity | 46.5 pg/mL | ||||||||||||||||||||||||
| Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
| Gene Symbol | AMSH |
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| Alias Symbols | Alpha-Melanotropin |
| Protein Name | Alpha-Melanocyte Stimulating Hormone |
| Description of Target | Alpha-Melanotropin is a 13-amino acid peptide derived from proteolytic cleavage of ADRENOCORTICOTROPIC HORMONE, the N-terminal segment of ACTH. ACTH (1-13) is amidated at the C-terminal to form ACTH (1-13)NH2 which in turn is acetylated to form alpha-MSH in the secretory granules. Alpha-MSH stimulates the synthesis and distribution of MELANIN in MELANOCYTES in mammals and MELANOPHORES in lower vertebrates. |
| Uniprot ID | O95630 |