Albumin ELISA Kit (Monkey)
Cat# OKIA00183
Size : 1x96wellplate
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Albumin ELISA Kit (Monkey) (OKIA00183)
| Datasheets/Manuals | Printable datasheet for Albumin ELISA Kit (Monkey) (OKIA00183) |
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| COA Datasheet | Printable COA datasheet for OKIA00183 |
| Predicted Species Reactivity | Monkey | ||||||||||||||||
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| Application | ELISA | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||
| ELISA Kit Duration | 100 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Albumin ELISA Kit (Monkey) (OKIA00183) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum albumin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum albumin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum albumin captured in the well. | ||||||||||||||||
| ELISA Kit Range | 12.5 ng/ml - 400 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The Albumin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Monkey. | ||||||||||||||||
| :: | REAGENTS 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ïL of affinity purified anti- Albumin antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI- ALBUMIN ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Albumin antibodies. 7. MONKEY ALBUMIN CALIBRATOR One vial containing Monkey Albumin calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 1.795 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Monkey | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
| Introduction | Albumin (Alb) is an amazing polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids. |
| Alias Symbols | Albumin, ALB |
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| NCBI Gene Id | 102130757 |
| Protein Name | Serum albumin |
| Description of Target | Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. |
| Uniprot ID | A2V9Z4 |