Adalimumab (Humira) Pharmacokinetic ELISA
Cat# EL-1611-011
Size : 1X96wells
Brand : AffinityImmuno
Contact local distributor :
Phone : +1 850 650 7790
This Adalimumab (Humira®) ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity, through use of a pair of antibodies.
Introduction
Adalimumab (Humira®) is a human monoclonal antibody used to block the action of tumor necrosis factor alpha (TNF-α). TNF-α is a strong proinflammatory cytokine involved in normal immunity and also various autoimmune diseases.
The Adalimumab (Humira®) ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices.
Principal of the Essay
This assay employs the sandwich enzyme immunoassay technique. Anti- Adalimumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adalimumab present in test samples.
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Catalog Number:
EL-1611-011 -
Application(s)
Measure free Adalimumab -
Target Protein
Humira -
Alternative Name of Target
Adalimumab -
Size
96 wells -
Sensitivity
Detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
1.5ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
Product Specifications
-
Catalog Number:
EL-1611-011 -
Application(s)
Measure free Adalimumab -
Target Protein
Humira -
Alternative Name of Target
Adalimumab -
Size
96 wells -
Sensitivity
Detection limit 1.5 ng/mL -
Detection Method
Peroxidase / OD450 -
Precision
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. -
Detection Limit
1.5ng/ml -
Storage Conditions
Stable at -20°C for 1 year -
Safety Warnings and Precautions
Research Use Only. Follow instructions. -
Each Kit Includes
Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3