11-Dehydrothromboxane B2 ELISA Kit
Cat# OKEH02578
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology 11-Dehydrothromboxane B2 ELISA Kit (OKEH02578) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-11-Dehydrothromboxane B2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 11-Dehydrothromboxane B2 and incubated. The 11-Dehydrothromboxane B2 found in the sample or standards competes with the biotinylated 11-Dehydrothromboxane B2 for limited binding sites on the immobilized anti-11-Dehydrothromboxane B2 antibody. Excess unbound biotinylated 11-Dehydrothromboxane B2 and sample or standard 11-Dehydrothromboxane B2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 11-Dehydrothromboxane B2 captured in the well and inversely proportional to the amount of 11-Dehydrothromboxane B2 which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 15.6-1000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 91% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.1% (n = 20) Mean Inter-assay CV%: < 8.5% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: 11DHTXB2 Target Name: 11-Dehydrothromboxane B2 | ||||||||||||||||||||||
| :: | Pubchem: 5280891 | ||||||||||||||||||||||
| :: | Chemical formula: C20H32O6 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 5.5 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General 11-Dehydrothromboxane B2 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | 11DHTXB2, 11-Dehydrothromboxane B2, 11DHTXB2 |
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| Protein Name | 11-Dehydrothromboxane B2 |
| Description of Target | 11-Dehydrothromboxane B2 (or 11-dehydro-TXB2) is produced from the breakdown of thromboxane A2. It is released by activated platelets and urine levels of 11-dehydro-TXB2 can be used to monitor the response to aspirin therapy when used to prevent heart disease and in diseases where platelet activation is prominent. |