Reagents and instruments for immunology, cell biology and molecular biology.
 
> 10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) (4 x 1 liter)

10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) (4 x 1 liter)

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Cat# MB-008-4000

Size : 4x1L

Brand : Rockland Immunochemicals

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Phone : +1 850 650 7790

Background

Phosphate buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

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Specifications for 10X PBS pH 7.2

Product Details

10X PBS pH 7.2 (0.2 M Potassium Phosphate 1.5 M Sodium Chloride) - MB-008
Phosphate buffered saline, Phosphate buffered solution, PBS, 10X PBS

Target Details

10X PBS buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Application Details

FC, IF, Other  - View References
This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium Chloride, pH 7.2 prepared in highly polished pharmaceutical grade water (WFI).

Formulation

Liquid
10X N/A
See application note.
None
None

Shipping & Handling

Ambient
Store container at room temperature (18° to 26° C) prior to opening. If desired, the solution may be stored at 4° C or less. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.
Expiration date is six (6) months from date of receipt.

References (25)

(). Distinctive features of SARS-CoV-2-specific T cells predict recovery from severe COVID-19. medRxiv.
Applications
Other
PubMed
(). Single-cell analyses of Crohn's disease tissues reveal intestinal intraepithelial T cells heterogeneity and altered subset distributions. Nat Commun.
Applications
FC, FACS, FLOW
PubMed
(). Immune Profiling Mass Cytometry Assay Harmonization: Multicenter Experience from CIMAC-CIDC. Clinical Cancer Research : An Official Journal of the American Association for Cancer Research
Applications
Buffer
PubMed
(). Bifidobacteria-mediated immune system imprinting early in life. Cell.
Applications
FC, FACS, FLOW
PubMed
(). Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4+ T cell subsets in vivo. Cell Rep.
Applications
FC, FACS, FLOW
PubMed
(). Lower PDL1, PDL2, and AXL Expression on Lung Myeloid Cells Suggests Inflammatory Bias in Smoking and Chronic Obstructive Pulmonary Disease. Am J Respir Cell Mol Biol.
Applications
FC, FACS, FLOW
PubMed
(). Human immune system variation during 1 year. Cell Rep.
Applications
FC, FACS, FLOW
PubMed
(). High-parameter immune profiling with CyTOF. Methods Mol Biol.
Applications
FC, FACS, FLOW
PubMed
(). Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy. Cell Rep.
Applications
FC, FACS, FLOW
PubMed
(). Systems-Level Immunomonitoring from Acute to Recovery Phase of Severe COVID-19 Cell Rep Med.
Applications
FC, FACS, FLOW
PubMed
(). SARS-CoV-2-specific T cells exhibit phenotypic features of helper function, lack of terminal differentiation, and high proliferation potential. Cell Rep Med.
Applications
FC, FACS, FLOW
PubMed
(). Profiling of the Human Natural Killer Cell Receptor-Ligand Repertoire. J Vis Exp.
Applications
FC, FACS, FLOW
PubMed
(). Immune profiling of human gut-associated lymphoid tissue identifies a role for isolated lymphoid follicles in priming of region-specific immunity. Immunity.
Applications
FC, FACS, FLOW; IF, Confocal Microscopy
PubMed
(). Mass cytometry reveals global immune remodeling with multi-lineage hypersensitivity to type I interferon in Down syndrome. Cell Rep.
Applications
FC, FACS, FLOW
PubMed
(). Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues. Nat Immunol.
Applications
FC, FACS, FLOW
PubMed
(). Gene regulatory programs conferring phenotypic identities to human NK cells. Cell.
Applications
FC, FACS, FLOW
PubMed
(). Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues. Nature Immunology
Applications
Buffer; FC, FACS, FLOW
PubMed
(). Stereotypic immune system development in newborn children. Cell.
Applications
FC, FACS, FLOW
PubMed
(). Comparison of CyTOF assays across sites: Results of a six-center pilot study. J Immunol Methods.
Applications
FC, FACS, FLOW
PubMed
(). Cytof measurement of immunocompetence across major immune cell types. Curr Protoc Cytom.
Applications
FC, FACS, FLOW
PubMed
(). Phenotyping of live human PBMC using CyTOFTM mass cytometry. Bio Protoc.
Applications
FC, FACS, FLOW
PubMed
(). Intracellular cytokine staining on PBMCs Using CyTOF™ mass cytometry. Bio Protoc.
Applications
FC, FACS, FLOW
PubMed
(). Cytokine-stimulated phosphoflow of PBMC using CyTOF mass cytometry. Bio Protoc.
Applications
FC, FACS, FLOW
PubMed
(). Cytokine-Stimulated Phosphoflow of Whole Blood Using CyTOF Mass Cytometry. Bio-Protocol
Applications
FC, FACS, FLOW; Buffer
PubMed
(). GENE EXPRESSION PATTERNS OF GAMMAHERPESVIRUSES. Olaf College
Applications
IF, Confocal Microscopy

Related Protocols

Flow Cytometry (FC) Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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