Tissue, Total RNA, Human Adult Normal, Testis, BioGenomics™

Cat# T5595-7403-50ug

Size : 50ug

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


T5595-7403 Rabbit Anti-Tissue, Total RNA, Human Adult Normal, Testis, BioGenomics™

Clone Type
Polyclonal
Grade
Molecular Biology Grade
Shipping Temp
Dry Ice
Storage Temp
-70°C

Total RNAs are available from almost 200 different human adult and fetal normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. Total RNAs are provided ready-to-use for Northern blotting, cDNA synthesis, RNA protection, and RNA differential display. |Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1/1 between 28S and 18S ribosomal RNA. We guarantee high efficiency reverse transcription.||Features:|• Total RNA isolated from a wide variety of hard to obtain tissues |• Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA |• Extensive quality control procedures to ensure high quality |• High efficiency reverse transcription |• Available documentation of tissues' clinical histories (additional cost)||Quality Control:|1. The integrity of the RNA is examined by visual inspectiuon for the presence of intact bands of 18s and 28s RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotomer. (A260/280 = 1.8-2.0 detected in 10mM Tris-Cl, pH 7.5).|2. The RNA is treated by DNAse I. It is tested as DNA free RNA by PCR.|3. cDNA synthesis is successfully performed by using this RNA as template.||Applications:|• Northern Blotting, RT-PCR, and RACE |• cDNA synthesis and cDNA library construction |• cDNA probe for profiling study in gene expression |• RNA protection and primer extension |• RNA display |• Purification of mRNA||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -70°C. Aliquots are stable for at least 6months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Purity: Total RNA isolated by modified guanidine thiocyanate techniques.|Concentration: ~2-3mg/ml|Form: Supplied as a liquid in RNA Storage Buffer: 0.1% DEPC-treated water with 0.1mM EDTA. No non-specific endonucleases, no nickase, no exonuclease and no RNase detected.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in RNA Storage Buffer: 0.1% DEPC-treated water with 0.1mM EDTA. No non-specific endonuleases, no nickase, no exonuclease and no RNase detected.
Purity
Total RNA isolated by modified guanidine thiocyanate techniques.

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